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Rabbit Recombinant Monoclonal Ki67 antibody. Suitable for mIHC, ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 22 publications.


Images

Western blot - Anti-Ki67 antibody [SP6] - BSA free (AB231172), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [SP6] - BSA free (AB231172), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ki67 antibody [SP6] - BSA free (AB231172), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA free (AB231172), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] - BSA free (AB231172), expandable thumbnail

Publications

  • Nature medicine 29:2939-29532023
    An integrated gene-to-outcome multimodal database for metabolic dysfunction-associated steatotic liver disease.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Timothy J Kendall,Maria Jimenez-Ramos,Frances Turner,Prakash Ramachandran,Jessica Minnier,Michael D McColgan,Masood Alam,Harriet Ellis,Donald R Dunbar,Gabriele Kohnen,Prakash Konanahalli,Karin A Oien,Lucia Bandiera,Filippo Menolascina,Anna Juncker-Jensen,Douglas Alexander,Charlie Mayor,Indra Neil Guha,Jonathan A Fallowfield
    PubMed 37903863
  • Nature communications 13:62032022
    Remodelling of tumour microenvironment by microwave ablation potentiates immunotherapy of AXL-specific CAR T cells against non-small cell lung cancer.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Bihui Cao,Manting Liu,Lu Wang,Kangshun Zhu,Mingyue Cai,Xiaopei Chen,Yunfei Feng,Shuo Yang,Shengyu Fu,Cheng Zhi,Xiaodie Ye,Jian Zhang,Zhiru Zhang,Xin Yang,Ming Zhao,Qingde Wu,Linfeng Xu,Lili Yang,Hui Lian,Qi Zhao,Zhenfeng Zhang
    PubMed 36261437

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCICC/IFWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Expected
Tested
Expected
Common marmoset
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Use at an assay dependent concentration.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Use at an assay dependent concentration.

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6)

Species

Rat

Dilution info

-

Notes

Use at an assay dependent concentration.

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6)

Species

Human

Dilution info

-

Notes

Use at an assay dependent concentration.

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6)

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Rabbit monoclonal isotype, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Common marmoset

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

    7 products for Alternative Product

      Target data

      Function

      Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

      Alternative names

      Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

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      Rabbit Recombinant Monoclonal Ki67 antibody. Suitable for mIHC, ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 22 publications.

      Alternative names

      Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      SP6

      Purification technique

      Affinity purification

      Epitope

      C-terminus

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab231172 is the carrier-free version of Anti-Ki67 antibody [SP6] ab16667.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

      Biological function summary

      The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

      Pathways

      Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

      Associated diseases and disorders

      Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com