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Rabbit Recombinant Monoclonal Ki67 antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 31 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6], prediluted (AB21700), expandable thumbnail
  • Western blot - Anti-Ki67 antibody [SP6], prediluted (AB21700), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [SP6], prediluted (AB21700), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ki67 antibody [SP6], prediluted (AB21700), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ki67 antibody [SP6], prediluted (AB21700), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

Use at an assay dependent concentration.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min

Expected
Expected

Species

Mouse

Dilution info

-

Notes

Use at an assay dependent concentration.

If fixing cells in 4% PFA (20 min, room temp), it is recommended to permeabilized cells with 0.1% Triton-X for 5 min

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1

Notes

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Expected
Expected

Species

Mouse

Dilution info

1/1

Notes

Antigen retrieval: heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

4 products for Alternative Version

Target data

Function

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

Recommended products

Rabbit Recombinant Monoclonal Ki67 antibody. Suitable for ICC/IF, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 31 publications.

Alternative names

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP6

Purification technique

Affinity purification Protein A

Specificity

ab21700 recognises Ki67.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

This product was switched from hybridoma to recombinant format on 25th October 2019.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.

Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Associated diseases and disorders

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com