Anti-KIAA0090 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-KIAA0090 antibody (AB242090)

KIAA0090 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242090 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab242090 at 1/250 dilution.

Lane 1 : ab242090 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-KIAA0090 antibody (ab242090)

Predicted band size: 112 kDa

false

• WB

Supplier Data

Western blot - Anti-KIAA0090 antibody (AB242090)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-KIAA0090 antibody (ab242090) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 112 kDa

true

Exposure time: 3min