AB242112

Anti-KIAA0090 antibody

Name: Anti-KIAA0090 antibody (ab242112) | Abcam
Brand: Abcam Limited
SKU: AB242112
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal KIAA0090 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EMC1 aa 1-50.

View Alternative Names

KIAA0090, PSEC0263, EMC1, ER membrane protein complex subunit 1

2 Images

Supplier Data

Immunoprecipitation - Anti-KIAA0090 antibody (AB242112)

KIAA0090 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab242112 used for IP at 20 μl per reaction. For WB at 1/250 dilution.

Lane 1 : ab242112 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG in Jurkat whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-KIAA0090 antibody (ab242112)

Predicted band size: 112 kDa

false

Supplier Data

Western blot - Anti-KIAA0090 antibody (AB242112)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-KIAA0090 antibody (ab242112) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 112 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human EMC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8N766

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "20 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to KIAA0090 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835, PubMed : 32439656, PubMed : 32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed : 29809151, PubMed : 30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed : 29242231, PubMed : 29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed : 30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).

See full target information EMC1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 18:4853-4868 PubMed35982901

2022

A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Yuan,Zitong Zhao,Zichan Guo,Liying Ma,Jing Han,Yongmei Song

eLife 9: PubMed33236988

2020

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshmi E Miller-Vedam,Bastian Bräuning,Katerina D Popova,Nicole T Schirle Oakdale,Jessica L Bonnar,Jesuraj R Prabu,Elizabeth A Boydston,Natalia Sevillano,Matthew J Shurtleff,Robert M Stroud,Charles S Craik,Brenda A Schulman,Adam Frost,Jonathan S Weissman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIAA0090 antibody

Immunoprecipitation - Anti-KIAA0090 antibody (AB242112)

Western blot - Anti-KIAA0090 antibody (AB242112)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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