AB226255

Anti-KIAA0101 antibody

Name: Anti-KIAA0101 antibody (ab226255) | Abcam
Brand: Abcam Limited
SKU: AB226255
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal KIAA0101 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PCLAF aa 1-50.

View Alternative Names

KIAA0101, NS5ATP9, PAF, L5, PCLAF, PCNA-associated factor, Hepatitis C virus NS5A-transactivated protein 9, Overexpressed in anaplastic thyroid carcinoma 1, PCNA-associated factor of 15 kDa, PCNA-clamp-associated factor, HCV NS5A-transactivated protein 9, OEATC-1, PAF15, p15PAF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0101 antibody (AB226255)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0101 antibody (AB226255)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KIAA0101 using ab226255 at 1/1000 dilution in immunohistochemical analysis.

Detection : DAB staining. Counterstain : Hematoxylin (blue).

Supplier Data

Immunoprecipitation - Anti-KIAA0101 antibody (AB226255)

KIAA0101 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226255 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226255 at 1 μg/ml.

Lane 1 : ab226255 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-KIAA0101 antibody (ab226255)

Predicted band size: 12 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human PCLAF aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226255 was affinity purified using an epitope specific to PAF immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.

See full target information PCLAF

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 13:1751-1762 PubMed35524161

2022

Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR-383-5p and regulating KIAA0101.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguang Sun,Shaowei Zhang,Nan Zhang,Jianxin Wang,Jindong Wang,Junfeng Liu

Oncology reports 46: PubMed34296306

2021

Circular RNA TADA2A promotes proliferation and migration via modulating of miR‑638/KIAA0101 signal in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Zhang,Hongmin Yao,Ying Li,Lu Yang,Liang Zhang,Jinxin Chen,Yong Wang,Xia Li

Experimental and therapeutic medicine 22:969 PubMed34335911

2021

Curcumin inhibits prostate cancer progression by regulating the miR-30a-5p/PCLAF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Pan,Jian Sha,Wenyao Lin,Yuxiong Wang,Tingzhang Bian,Jianming Guo

Cell cycle (Georgetown, Tex.) 20:1010-1020 PubMed33970778

2021

PAF enhances cancer stem cell properties via β-catenin signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Li,Xu-Dong Mu,Juan-Rong Song,Peng-Tao Zhai,Yuan Cheng,Yao Le,Zhu-Bin Li

Molecular cell 81:1698-1714.e6 PubMed33626321

2021

PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Moon Jong Kim,Christopher Cervantes,Youn-Sang Jung,Xiaoshan Zhang,Jie Zhang,Sung Ho Lee,Sohee Jun,Larisa Litovchick,Wenqi Wang,Junjie Chen,Bingliang Fang,Jae-Il Park

Biochemical and biophysical research communication 503:600-606 PubMed29902451

2018

KIAA0101 inhibition suppresses cell proliferation and cell cycle progression by promoting the interaction between p53 and Sp1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lv,Benhua Su,Yuyang Li,Chong Geng,Na Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIAA0101 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0101 antibody (AB226255)

Immunoprecipitation - Anti-KIAA0101 antibody (AB226255)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise