AB201467

Anti-KIAA0652/ATG13 antibody [EPR19601]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal KIAA0652/ATG13 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

KIAA0652, ATG13, Autophagy-related protein 13

6 Images

Flow Cytometry (Intracellular) - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling KIAA0652/ATG13 with ab201467 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.

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Immunoprecipitation - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

KIAA0652/ATG13 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201467 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201467 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10μg (Input).

Lane 2 : ab201467 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab201467 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467)

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 56 kDa

Observed band size: 70 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 70 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1-3 : 30 seconds; Lane 4 : 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID : 18936157).

All lanes:

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 20 µg

Lane 4:

A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 70 kDa

false

Supplier Data

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (ab201467) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 5:

Mouse brain tissue lysate at 10 µg

Lane 6:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 70 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19601

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

See full target information ATG13

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 16:1170313 PubMed37138705

2023

Peroxisomal defects in microglial cells induce a disease-associated microglial signature.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Raas,Ali Tawbeh,Mounia Tahri-Joutey,Catherine Gondcaille,Céline Keime,Romain Kaiser,Doriane Trompier,Boubker Nasser,Valerio Leoni,Emma Bellanger,Maud Boussand,Yannick Hamon,Alexandre Benani,Francesca Di Cara,Caroline Truntzer,Mustapha Cherkaoui-Malki,Pierre Andreoletti,Stéphane Savary

International journal of molecular sciences 23: PubMed35008820

2021

Transport and Toxicity of Methylmercury-Cysteine in Cultured BeWo Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Srividya Ganapathy,Elisa R Farrell,Simran Vaghela,Lucy Joshee,Earl G Ford,Olga Uchakina,Robert J McKallip,Jennifer L Barkin,Christy C Bridges

EMBO reports 22:e51136 PubMed34369648

2021

BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.

Applications

Unspecified application

Species

Unspecified reactive species

Ashari R Kannangara,Daniel M Poole,Colten M McEwan,Joshua C Youngs,Vajira K Weerasekara,Alex M Thornock,Misael T Lazaro,Eranga R Balasooriya,Laura M Oh,Erik J Soderblom,Jonathan J Lee,Daniel L Simmons,Joshua L Andersen

Scientific reports 11:14898 PubMed34290273

2021

Interleukin-27 promotes autophagy in human serum-induced primary macrophages via an mTOR- and LC3-independent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvain Laverdure,Ziqiu Wang,Jun Yang,Takuya Yamamoto,Tima Thomas,Toyotaka Sato,Kunio Nagashima,Tomozumi Imamichi

Nature 589:456-461 PubMed33328639

2020

Sorting nexin 5 mediates virus-induced autophagy and immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Dong,Yuting Yang,Zhongju Zou,Yuting Zhao,Bo Ci,Lin Zhong,Madhura Bhave,Liwei Wang,Yi-Chun Kuo,Xiao Zang,Rui Zhong,Elizabeth R Aguilera,R Blake Richardson,Boris Simonetti,John W Schoggins,Julie K Pfeiffer,Li Yu,Xuewu Zhang,Yang Xie,Sandra L Schmid,Guanghua Xiao,Paul A Gleeson,Nicholas T Ktistakis,Peter J Cullen,Ramnik J Xavier,Beth Levine

Nature methods 17:232-239 PubMed31768061

2019

An antibody for analysis of autophagy induction.

Applications

Unspecified application

Species

Unspecified reactive species

Wensheng Tian,Reham Alsaadi,Zhihao Guo,Alena Kalinina,Micaël Carrier,Marie-Eve Tremblay,Baptiste Lacoste,Diane Lagace,Ryan C Russell

Experimental and therapeutic medicine 18:2574-2582 PubMed31555366

2019

Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Zou,Xiaokun Ma,Shuo Lin,Bingyuan Wu,Yang Chen,Chaoquan Peng

Molecular medicine reports 19:3519-3526 PubMed30896869

2019

Overexpression of ribonuclease inhibitor induces autophagy in human colorectal cancer cells via the Akt/mTOR/ULK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Tang,Feng Ren,Xi Cong,Ying Kong,Yuxiang Tian,Yuefei Xu,Jianhui Fan

International journal of molecular medicine 41:2086-2098 PubMed29344647

2018

Autophagy regulates the degeneration of the auditory cortex through the AMPK-mTOR-ULK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yuan,Xueyan Zhao,Yujuan Hu,Haiying Sun,Guoqing Gong,Xiang Huang,Xubo Chen,Mingyu Xia,Chen Sun,Qilin Huang,Yu Sun,Wen Kong,Weijia Kong

Cell death & disease 7:e2563 PubMed28032867

2016

Regulation of ER stress-induced autophagy by GSK3β-TIP60-ULK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tiejian Nie,Shaosong Yang,Hongwei Ma,Lei Zhang,Fangfang Lu,Kai Tao,Ronglin Wang,Ruixin Yang,Lu Huang,Zixu Mao,Qian Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIAA0652/ATG13 antibody [EPR19601]

Flow Cytometry (Intracellular) - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Immunoprecipitation - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Western blot - Anti-KIAA0652/ATG13 antibody [EPR19601] (AB201467)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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