Rabbit Recombinant Monoclonal KIAA1199 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
Images
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409552.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)")
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409550.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)")
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409551.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)")
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409553.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.
Alternative names
HYBID, KIAA1199, CEMIP, Cell migration-inducing and hyaluronan-binding protein, Hyaluronan binding protein involved in hyaluronan depolymerization
Recommended products
Rabbit Recombinant Monoclonal KIAA1199 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27442-24
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409552.jpg "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)")
Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)
This data was developed using Anti-KIAA1199 antibody [EPR27442-24] ab308288, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Frozen lysates were used for Western blotting.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsLane 1: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HCT116 transfected with siRNA specifically targeti KIAA1199 whole cell lysate
Observed band size: 150 kDa
Exposure time: 180s
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409550.jpg "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)")
Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)
This data was developed using Anti-KIAA1199 antibody [EPR27442-24] ab308288, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human CEMIP2.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 15 secondsLane 1: 293T cells transfected with an empty vector containi a his tag whole cell lysate
Lane 2: 293T cells transfected with a human KIAA1199 expression vector containi a his tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human CEMIP2 expression vector containi a his tag whole cell lysate
Observed band size: 150 kDa
Exposure time: 15s
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409551.jpg "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)")
Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)
This data was developed using Anti-KIAA1199 antibody [EPR27442-24] ab308288, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: 293T (PMID: 33473125), RPMI-8226, U937.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-KIAA1199 antibody [EPR27442-24] (Anti-KIAA1199 antibody [EPR27442-24] ab308288) at 1/1000 dilution
Lane 1: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3: RPMI-8226 (human plasmacytoma, myeloma B lymphocyte ) whole cell lysate
Lane 4: U937 (human histiocytic lymphoma monocyte) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa
Exposure time: 180s
![Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291), expandable thumbnail](https://content.abcam.com/products/images/kiaa1199-antibody-epr27442-24-bsa-and-azide-free-ab308291--western-blot-img409553.jpg "Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)")
Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (ab308291)
This data was developed using Anti-KIAA1199 antibody [EPR27442-24] ab308288, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: MCF7 (PMID: 31303964).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsLane 1: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Observed band size: 150 kDa
Exposure time: 180s
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