Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)

This data was developed using ab308288, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : 293T (PMID : 33473125), RPMI-8226, U937. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-KIAA1199 antibody [EPR27442-24] (<a href='/en-us/products/primary-antibodies/kiaa1199-antibody-epr27442-24-ab308288'>ab308288</a>) at 1/1000 dilution

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

RPMI-8226 (human plasmacytoma, myeloma B lymphocyte ) whole cell lysate

Lane 4:

U937 (human histiocytic lymphoma monocyte) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)

This data was developed using ab308288, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : MCF7 (PMID : 31303964). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

Lane 1:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

Observed band size: 150 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)

This data was developed using ab308288, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Frozen lysates were used for Western blotting. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HCT116 transfected with siRNA specifically targeti KIAA1199 whole cell lysate

Observed band size: 150 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KIAA1199 antibody [EPR27442-24] - BSA and Azide free (AB308291)

This data was developed using ab308288, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human CEMIP2. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

Lane 1:

293T cells transfected with an empty vector containi a his tag whole cell lysate

Lane 2:

293T cells transfected with a human KIAA1199 expression vector containi a his tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human CEMIP2 expression vector containi a his tag whole cell lysate

Observed band size: 150 kDa

false

Exposure time: 15s