Anti-KIAA1199 antibody [EPR30046-538]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIAA1199 antibody [EPR30046-538] (AB324386)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized KATO III (human stomach spherical) cells labelling KIAA1199 with ab324386 at 1/500 (0.988 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in KATO III cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : MCF7 (PMID : 31303964)
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIAA1199 antibody [EPR30046-538] (AB324386)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized COLO 201 (human derived from metastatic site ascites bipolar, slightly refractile, fibroblast-like cell) cells labelling KIAA1199 with ab324386 at 1/500 (0.988 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in COLO 201 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : U-937.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Dot

Supplier Data

Dot Blot - Anti-KIAA1199 antibody [EPR30046-538] (AB324386)

Dot blot analysis of KIAA1199 using ab324386 at 1 : 1000 (0.494 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with a human CEMIP expression vector containing a His-tag whole cell lysate
Lane2 : 293T cells transfected with a human CEMIP2 expression vector containing a His-tag whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-KIAA1199 antibody [EPR30046-538] (ab324386) at 1/1000 dilution

Lane 1:

293T cells transfected with a human CEMIP expression vector containing a His-tag whole cell lysate

Lane 2:

293T cells transfected with a human CEMIP2 expression vector containing a His-tag whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

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