Anti-KIAA1279 antibody [EPR30401-526]

7 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Immunocytochemistry/ Immunofluorescence staining of F9 (mouse embryonal carcinoma epithelial cell) using rabbit Anti-KIAA1279 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling KIAA1279 with ab324162 at 1/200 (2.535 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplamic staining in F9 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: ab324162 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Immunocytochemistry/ Immunofluorescence staining of Mouse splenocyte using rabbit Anti-KIAA1279 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocyte cells labelling KIAA1279 with ab324162 at 1/200 (2.535 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Low expression: Confocal image showing negative staining in mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  

  Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: ab324162 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Immunocytochemistry/ Immunofluorescence staining of Mouse primary neural/glia cell using rabbit Anti-KIAA1279 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling KIAA1279 with ab324162 at 1/200 (2.535 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: ab324162 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-KIAA1279 antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labelling KIAA1279 with ab324162 at 1/200 (2.535 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in HEK-293 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Low expression: THP-1.

  Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: ab324162 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
  -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

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  Western blot - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Western blot staining using rabbit Anti-KIAA1279 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: liver, lung, pancreas, spleen (PMID: 15883926).

  The identity of the bands lower than 37 kDa are unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-KIAA1279 antibody [EPR30401-526] (ab324162) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 50 µg

  Lane 2: Mouse lung tissue lysate at 50 µg

  Lane 3: Mouse liver tissue lysate at 50 µg

  Lane 4: Mouse pancreas tissue lysate at 50 µg

  Lane 5: Mouse testis tissue lysate at 50 µg

  Lane 6: Mouse spleen tissue lysate at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 75 kDa, 36 kDa

  Exposure time: 37s

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  Western blot - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Western blot staining using rabbit Anti-KIAA1279 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: THP-1, Daudi.

  The identity of the band at approximately 30 kDa is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-KIAA1279 antibody [EPR30401-526] (ab324162) at 1/1000 dilution

  Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

  Lane 2: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 50 µg

  Lane 3: Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 50 µg

  Lane 4: Mouse primary neuron cell lysate at 50 µg

  Lane 5: C2C12 (mouse myoblast) whole cell lysate at 50 µg

  Lane 6: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 50 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 75 kDa, 36 kDa

  Exposure time: 15s

• 

  Western blot - Anti-KIAA1279 antibody [EPR30401-526] (ab324162)

  KIAA1279 Western blot staining using rabbit Anti-KIAA1279 antibody

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the band at approximately 30 kDa is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-KIAA1279 antibody [EPR30401-526] (ab324162) at 1/1000 dilution

  Lane 1: HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

  Lane 2: HEK-293 transfected with siRNA specifically targeting KIAA1279 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 75 kDa, 36 kDa

  Exposure time: 180s