AB216508

Anti-KIBRA antibody

Name: Anti-KIBRA antibody (ab216508) | Abcam
Brand: Abcam Limited
SKU: AB216508
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal KIBRA antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human WWC1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0869, KIBRA, WWC1, Protein KIBRA, HBeAg-binding protein 3, Kidney and brain protein, WW domain-containing protein 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIBRA antibody (AB216508)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIBRA antibody (AB216508)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling KIBRA with ab216508 1/200 dilution, followed by secondary antibody detection and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIBRA antibody (AB216508)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling KIBRA with ab216508 1/200 dilution, followed by secondary antibody detection and DAB staining.

Lab

Western blot - Anti-KIBRA antibody (AB216508)

All lanes:

Western blot - Anti-KIBRA antibody (ab216508) at 1/1000 dilution

Lane 1:

Siha cell lysates

Lane 2:

A431 cell lysates

Lane 3:

MCF-7 cell lysates

Lane 4:

U2OS cell lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/20000 dilution

Predicted band size: 125 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human WWC1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8IX03

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of the Hippo signaling pathway, also known as the Salvador-Warts-Hippo (SWH) pathway (PubMed : 24682284). Enhances phosphorylation of LATS1 and YAP1 and negatively regulates cell proliferation and organ growth due to a suppression of the transcriptional activity of YAP1, the major effector of the Hippo pathway (PubMed : 24682284). Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and function in the regulation of Hippo signaling pathway (PubMed : 20159598). Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation (PubMed : 16684779). Regulates collagen-stimulated activation of the ERK/MAPK cascade (PubMed : 18190796). Modulates directional migration of podocytes (PubMed : 18596123). Plays a role in cognition and memory performance (PubMed : 18672031). Plays an important role in regulating AMPA-selective glutamate receptors (AMPARs) trafficking underlying synaptic plasticity and learning (By similarity).

See full target information WWC1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl0030 PubMed38924398

2024

KIBRA anchoring the action of PKMζ maintains the persistence of memory.

Applications

Unspecified application

Species

Unspecified reactive species

Panayiotis Tsokas,Changchi Hsieh,Rafael E Flores-Obando,Matteo Bernabo,Andrew Tcherepanov,A Iván Hernández,Christian Thomas,Peter J Bergold,James E Cottrell,Joachim Kremerskothen,Harel Z Shouval,Karim Nader,André A Fenton,Todd C Sacktor

Diagnostics (Basel, Switzerland) 12: PubMed36552980

2022

Expression of the Hippo Pathway Core Components in Endometrial Cancer and Its Association with Clinicopathologic Features.

Applications

Unspecified application

Species

Unspecified reactive species

Juseok Yang,Dae Hyun Song,Cho Hee Kim,Min Hye Kim,Hyen Chul Jo,Hyoeun Kim,Ji Eun Park,Jong Chul Baek

Molecular therapy : the journal of the American Society of Gene Therapy 30:3313-3332 PubMed35619557

2022

Neat1 promotes acute kidney injury to chronic kidney disease by facilitating tubular epithelial cells apoptosis via sequestering miR-129-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Tongtong Ma,Hongwei Li,Hui Liu,Yili Peng,Tong Lin,Zhiya Deng,Nan Jia,Zhongqing Chen,Peng Wang

Molecular medicine reports 24: PubMed34505633

2021

Inhibin βA is an independent prognostic factor that promotes invasion via Hippo signaling in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yijun Zhang,Shumei Yan,Yan Li,Jiangbo Zhang,Yuan Luo,Pengcheng Li,Yuanzhong Yang,Yong Li,Yuhua Huang,Enhua Wang

The Kaohsiung journal of medical sciences 38:6-17 PubMed34431595

2021

Long non-coding RNA XIST alleviates sepsis-induced acute kidney injury through inhibiting inflammation and cell apoptosis via regulating miR-155-5p/WWC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Qiu-Mei Cao

BMC medicine 18:8 PubMed31959160

2020

Profiling of the muscle-specific dystroglycan interactome reveals the role of Hippo signaling in muscular dystrophy and age-dependent muscle atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Andriy S Yatsenko,Mariya M Kucherenko,Yuanbin Xie,Dina Aweida,Henning Urlaub,Renate J Scheibe,Shenhav Cohen,Halyna R Shcherbata

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIBRA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIBRA antibody (AB216508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIBRA antibody (AB216508)

Western blot - Anti-KIBRA antibody (AB216508)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise