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AB97345

Anti-Kidins220 antibody

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(1 Publication)

Rabbit Polyclonal Kidins220 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KIDINS220 aa 1700 to C-terminus.

View Alternative Names

ARMS, KIAA1250, KIDINS220, Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kidins220 antibody (AB97345)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kidins220 antibody (AB97345)

ab97345 at 1/500 dilution staining Kidins220 in paraffin-embedded SW480 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Western blot - Anti-Kidins220 antibody (AB97345)
  • WB

Unknown

Western blot - Anti-Kidins220 antibody (AB97345)

5% SDS PAGE

All lanes:

Western blot - Anti-Kidins220 antibody (ab97345) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Predicted band size: 197 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human KIDINS220 aa 1700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9ULH0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kidins220 also known as Ankyrin Repeat-rich Membrane Spanning (ARMS) is a scaffold protein that plays a significant role in cellular signaling. It has a molecular mass of approximately 220 kDa. Primarily found in neurons Kidins220 is expressed in the central and peripheral nervous systems indicating its importance in neural processes. The presence of ankyrin repeats and transmembrane helices in its structure allows Kidins220 to interact with a variety of proteins facilitating multiple signaling functions in cells.
Biological function summary

Kidins220 is important in the regulation of neuronal development and survival. It forms part of a larger signaling complex that includes Trk and Eph receptors which are critical for nerve growth and cell differentiation. Kidins220 interacts with these receptors to mediate cellular responses to growth factors like nerve growth factor (NGF). It supports neuronal outgrowth and shapes synapse formation by coordinating the intracellular signaling cascades necessary for these processes.

Pathways

Kidins220 actively participates in the MAPK and PI3K/Akt pathways essential for promoting cell survival and proliferation. In these pathways Kidins220 acts as a pivotal node linking membrane receptors to downstream signaling effectors. It associates with proteins such as TrkA engaging in signal transduction that modulates synaptic plasticity and cytoskeletal dynamics. By influencing these pathways Kidins220 aids in the maintenance of neuronal structure and function.

Aberrant Kidins220 function has associations with neurodegenerative diseases and cancers. Its dysregulation can lead to improper cell signaling and contribute to conditions like Alzheimer's disease and neuroblastoma. In the context of Alzheimer's Kidins220's interaction with proteins such as Amyloid Precursor Protein (APP) may influence amyloid-beta production. Additionally its role in neuroblastoma highlights its involvement in tumor development where it potentially alters the signaling processes that control cell proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.
See full target information KIDINS220
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:47444-47464 PubMed27329728

2016

MiR-4638-5p inhibits castration resistance of prostate cancer through repressing Kidins220 expression and PI3K/AKT pathway activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Ning Shao,Xueying Mao,Minmin Zhu,Weifei Fan,Zhixiang Shen,Rong Xiao,Chuncai Wang,Wenping Bao,Xinyu Xu,Chun Yang,Jian Dong,Deshui Yu,Yan Wu,Caixia Zhu,Liting Wen,Xiaojie Lu,Yong-Jie Lu,Ninghan Feng
View all publications
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