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Rabbit Recombinant Monoclonal KIF13A antibody. Suitable for Dot, ICC/IF, WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Dot Blot - Anti-KIF13A antibody [EPR29130-81] (AB323873), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF13A antibody [EPR29130-81] (AB323873), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF13A antibody [EPR29130-81] (AB323873), expandable thumbnail
  • Western blot - Anti-KIF13A antibody [EPR29130-81] (AB323873), expandable thumbnail
  • Western blot - Anti-KIF13A antibody [EPR29130-81] (AB323873), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotICC/IFWBIHC-FrIHC-P
Human
Expected
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abscission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis.

Alternative names

RBKIN, KIF13A, Kinesin-like protein KIF13A, Kinesin-like protein RBKIN

Rabbit Recombinant Monoclonal KIF13A antibody. Suitable for Dot, ICC/IF, WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29130-81
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for WB in human tissues.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Dot Blot - Anti-KIF13A antibody [EPR29130-81] (ab323873), expandable thumbnail

    Dot Blot - Anti-KIF13A antibody [EPR29130-81] (ab323873)

    KIF13A Dot Blot staining using rabbit Anti-KIF13A antibody

    This antibody does not cross-react with human KIF13B.

    #In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (Anti-6X His tag® antibody [AD1.1.10] ab15149) staining at 1/1000 dilution.

    All lanes: Dot Blot - Anti-KIF13A antibody [EPR29130-81] (ab323873) at 1/1000 dilution

    Lane 1: Purified 293T cells transfected with a human KIF13A expression vector containing a His-tag, whole cell lysate

    Lane 2: Purified 293T cells transfected with a human KIF13B expression vector containing a His-tag, whole cell lysate

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF13A antibody [EPR29130-81] (ab323873), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KIF13A antibody [EPR29130-81] (ab323873)

    KIF13A Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-KIF13A antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LN-229 (human brain glioblastoma epithelial cell) cells labelling KIF13A with ab323873 at 1/100 (5.29 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in LN-229 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: Daudi.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF13A antibody [EPR29130-81] (ab323873), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KIF13A antibody [EPR29130-81] (ab323873)

    KIF13A Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-KIF13A antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling KIF13A with ab323873 at 1/100 (5.29 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: Jurkat.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 1000 2ug/ml dilution.

  • Western blot - Anti-KIF13A antibody [EPR29130-81] (ab323873), expandable thumbnail

    Western blot - Anti-KIF13A antibody [EPR29130-81] (ab323873)

    KIF13A Western blot staining using rabbit Anti-KIF13A antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: THP-1; MOLT-4

    The molecular weight observed is consistent with what has been described in the literature (PMID: 33179593; PMID: 33179593).

    In Western blot, Anti-alpha Actinin 4 antibody [EPR2533 (2)](Anti-alpha Actinin 4 antibody [EPR2533(2)] - Loading Control ab108198) staining at 105KDa dilution.

    All lanes: Western blot - Anti-KIF13A antibody [EPR29130-81] (ab323873) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

    Lane 2: TF-1 (human erythroleukemia erythroblast) whole cell lysate at 40 µg

    Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg

    Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 250 kDa, 105 kDa

    Exposure time: 180s

  • Western blot - Anti-KIF13A antibody [EPR29130-81] (ab323873), expandable thumbnail

    Western blot - Anti-KIF13A antibody [EPR29130-81] (ab323873)

    KIF13A Western blot staining using rabbit Anti-KIF13A antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Daudi, Jurkat, U-937, HL-60.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 33179593; PMID: 33179593).

    In Western blot, Anti-alpha Actinin 4 antibody [EPR2533 (2)](Anti-alpha Actinin 4 antibody [EPR2533(2)] - Loading Control ab108198) staining at 105KDa dilution.

    All lanes: Western blot - Anti-KIF13A antibody [EPR29130-81] (ab323873) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: LN-229 (human brain glioblastoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 40 µg

    Lane 4: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 40 µg

    Lane 5: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 40 µg

    Lane 6: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 250 kDa, 105 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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