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AB251863

Anti-KIF18A antibody

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(2 Publications)

Rabbit Polyclonal KIF18A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KIF18A aa 500-650.

View Alternative Names

OK/SW-cl.108, KIF18A, Kinesin-like protein KIF18A, Marrow stromal KIF18A, MS-KIF18A

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)

Paraffin-embedded human fallopian tube tissue stained for KIF18A using ab251863 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KIF18A antibody (AB251863)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KIF18A antibody (AB251863)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for KIF18A (green) using ab251863 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)

Paraffin-embedded human kidney tissue stained for KIF18A using ab251863 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)

Paraffin-embedded human testis tissue stained for KIF18A using ab251863 at 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF18A antibody (AB251863)

Paraffin-embedded human rectum tissue stained for KIF18A using ab251863 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-KIF18A antibody (AB251863)
  • WB

Unknown

Western blot - Anti-KIF18A antibody (AB251863)

All lanes:

Western blot - Anti-KIF18A antibody (ab251863) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 102 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KIF18A aa 500-650. The exact immunogen used to generate this antibody is proprietary information.

Q8NI77

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.
See full target information KIF18A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1490781 PubMed39610707

2024

Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Flavia Serpico,Caterina Pisauro,Asia Trano,Domenico Grieco

Nature communications 12:6412 PubMed34741010

2021

Crosstalk between CST and RPA regulates RAD51 activity during replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Hang Lei,Han-Lin Yang,Hao-Yen Chang,Hsin-Yi Yeh,Dinh Duc Nguyen,Tzu-Yu Lee,Xinxing Lyu,Megan Chastain,Weihang Chai,Hung-Wen Li,Peter Chi
View all publications
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Product promise

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