AB72417

Anti-KIF18A antibody

Name: Anti-KIF18A antibody (ab72417) | Abcam
Brand: Abcam Limited
SKU: AB72417
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(8 Publications )

Rabbit Polyclonal KIF18A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human KIF18A.

2 Images

Unknown

Immunoprecipitation - Anti-KIF18A antibody (AB72417)

Detection of KIF18A by Western Blot of Immunprecipitate.
ab72417 at 1µg/ml staining KIF18A in HeLa whole cell lysate immunoprecipitated using ab72417 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-KIF18A antibody (ab72417)

Predicted band size: 102 kDa

false

Unknown

Western blot - Anti-KIF18A antibody (AB72417)

All lanes:

Western blot - Anti-KIF18A antibody (ab72417) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 102 kDa

Observed band size: 117 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human KIF18A. The exact immunogen used to generate this antibody is proprietary information.

Q8NI77

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72417 was affinity purified using an epitope specific to KIF18A immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.

See full target information KIF18A

Alternative Names

OK/SW-cl.108, KIF18A, Kinesin-like protein KIF18A, Marrow stromal KIF18A, MS-KIF18A

style

left-column

Publications (8)

Recent publications for all applications. Explore the full listand refine your search

The FEBS journal 292:3910-3925 PubMed39954259

2025

Kinesin-like protein KIF18A is required for faithful coordination of chromosome congression with cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Su Hyun Lee,Mi-Sun Kwon,Taerim Lee,Sungchul Hohng,Hyunsook Lee

Cancer biomarkers : section A of Disease markers 41:165-178 PubMed39331093

2024

KIF18A promotes cervical squamous cell carcinoma progression by activating the PI3K/AKT pathway through upregulation of CENPE.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyi Sun,Tiantian Zhao

Journal of Cancer 15:2260-2275 PubMed38495502

2024

Development and Validation of the novel Cuproptosis- and Immune-related Signature for Predicting Prognosis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yongping Zhang,Ping Sui,Cheng Zhong,Jiansheng Liu

Frontiers in oncology 13:1211103 PubMed37965453

2023

Development and validation of a novel anoikis-related gene signature in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Wu,Yuxiang Sun,Xiangcheng Qin,Maomao Li,Shuaishuai Huang,Xue Wang,Guobin Weng

Aging 15:9182-9192 PubMed37708299

2023

improves migration and invasion of colorectal cancer (CRC) cells through inhibiting signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liu,Ming Sun,Bin Zhang,Wenyan Zhao

Experimental and therapeutic medicine 25:154 PubMed36911368

2023

KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Yang,Qiaorong Zhang,Ziyuan Yang,Jiaming Shu,Lingling Zhang,Yangyang Yao,Xiaolang Wang,Xianxian Liu

Translational cancer research 8:2252-2263 PubMed35116978

2019

KIF18A promotes head and neck squamous cell carcinoma invasion and migration via activation of Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guancheng Liu,Gengming Cai,Xiaosong He,Donghai Huang,Gangcai Zhu,Changhan Chen,Xin Zhang

Experimental and therapeutic medicine 12:377-383 PubMed27347065

2016

Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Q I Chen,Bin Cao,Ning Nan,Y U Wang,X U Zhai,Youfang Li,Tie Chong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIF18A antibody

Immunoprecipitation - Anti-KIF18A antibody (AB72417)

Western blot - Anti-KIF18A antibody (AB72417)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (8)

Product promise