Anti-KIF1A antibody [EPR11790(B)]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with unpurified ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Intracellular Flow Cytometry analysis ofSH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling KIF1A with purifiedab180153 at 1/90 dilution (10 ug/ml).Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1/2000 dilution.Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabelled control, cells without incubation with primary antibody and secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedSH-SY5Y cells labeling KIF1A with unpurified ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling KIF1A with purified ab180153 at 1/100 dilution (9.2μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the secondary antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as the nuclear counter stain.

• IP

Lab

Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

ab180153 (purified) at 1/50 dilution (2ug) immunoprecipitating KIF1A in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

Lane 1 (input) : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

Lane 2 (+) : ab180153 + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab180153 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Predicted band size: 191 kDa

Observed band size: 191 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Western blot analysis of 293 cell lysate immunoprecipitated using unpurified ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] (ab180153)

Predicted band size: 191 kDa

false

• WB

Supplier Data

Western blot - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

All lanes:

Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/20000 dilution

All lanes:

293 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 191 kDa

false

• WB

Lab

Western blot - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 191 kDa

Observed band size: 191 kDa

false

• WB

Lab

Western blot - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/1000 dilution

Lane 1:

Rat hypothalamus lysate at 15 µg

Lane 2:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 15 kDa,191 kDa

Observed band size: 14 kDa,191 kDa

false

• WB

Supplier Data

Western blot - Anti-KIF1A antibody [EPR11790(B)] (AB180153)

All lanes:

Western blot - Anti-KIF1A antibody [EPR11790(B)] (ab180153) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 191 kDa

false