Rabbit Recombinant Monoclonal KIF1A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse, Recombinant fragment - Mouse, Rat samples.
Images
![Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (AB282820), expandable thumbnail](https://content.abcam.com/products/images/kif1a-antibody-epr24329-6-bsa-and-azide-free-ab282820--western-blot-img181723.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (AB282820)")
![Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (AB282820), expandable thumbnail](https://content.abcam.com/products/images/kif1a-antibody-epr24329-6-bsa-and-azide-free-ab282820--western-blot-img167872.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (AB282820)")
![Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (AB282820), expandable thumbnail](https://content.abcam.com/products/images/kif1a-antibody-epr24329-6-bsa-and-azide-free-ab282820--immunocytochemistry-immunofluorescence-img181257.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (AB282820)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Recombinant fragment - Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Recombinant fragment - Mouse, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Mouse, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Mouse, Human | Dilution info - | Notes - |
Target data
Function
Motor for anterograde axonal transport of synaptic vesicle precursors (Probable). Also required for neuronal dense core vesicles (DCVs) transport to the dendritic spines and axons. The interaction calcium-dependent with CALM1 increases vesicle motility and interaction with the scaffolding proteins PPFIA2 and TANC2 recruits DCVs to synaptic sites (By similarity).
Alternative names
Atsv, Kif1, Kif1a, Kinesin-like protein KIF1A, Axonal transporter of synaptic vesicles
Recommended products
Rabbit Recombinant Monoclonal KIF1A antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse, Recombinant fragment - Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24329-6
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab282820 is the carrier-free version of Anti-KIF1A antibody [EPR24329-6] ab271047.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820), expandable thumbnail](https://content.abcam.com/products/images/kif1a-antibody-epr24329-6-bsa-and-azide-free-ab282820--western-blot-img181723.jpg "Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820)")
Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820)
This data was developed using Anti-KIF1A antibody [EPR24329-6] ab271047, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: spleen, testis, kidney (PMID: 7539720).
Nonspecifc bands under 100 kDa are observed.
Exposure time: 48 seconds
All lanes: Western blot - Anti-KIF1A antibody [EPR24329-6] (Anti-KIF1A antibody [EPR24329-6] ab271047) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebral cortex tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse testis tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Rat brain tissue lysate at 20 µg
Lane 7: Rat spinal cord tissue lysate at 20 µg
Lane 8: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 191 kDa
Observed band size: 200 kDa
![Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820), expandable thumbnail](https://content.abcam.com/products/images/kif1a-antibody-epr24329-6-bsa-and-azide-free-ab282820--western-blot-img167872.jpg "Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820)")
Western blot - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820)
This data was developed using Anti-KIF1A antibody [EPR24329-6] ab271047, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 seconds
All lanes: Western blot - Anti-KIF1A antibody [EPR24329-6] (Anti-KIF1A antibody [EPR24329-6] ab271047) at 1/1000 dilution
Lane 1: His-tagged mouse KIF1A recombinant protein (aa2-355) at 0.01 µg
Lane 2: His-tagged mouse KIF1B recombinant protein (aa2-355) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 191 kDa
Observed band size: 37 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820), expandable thumbnail](https://content.abcam.com/products/images/kif1a-antibody-epr24329-6-bsa-and-azide-free-ab282820--immunocytochemistry-immunofluorescence-img181257.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820)")
Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR24329-6] - BSA and Azide free (ab282820)
This data was developed using Anti-KIF1A antibody [EPR24329-6] ab271047, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling KIF1A with Anti-KIF1A antibody [EPR24329-6] ab271047 at 1/50 (10.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing positive staining in mouse primary neuron.
Negative control: mouse spleen cell (PMID:7539720) is observed.
Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
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