AB70791

Anti-KIF20A antibody

Name: Anti-KIF20A antibody (ab70791) | Abcam
Brand: Abcam Limited
SKU: AB70791
Price: 525 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal KIF20A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human KIF20A.

View Alternative Names

MKLP2, RAB6KIFL, KIF20A, Kinesin-like protein KIF20A, GG10_2, Mitotic kinesin-like protein 2, Rab6-interacting kinesin-like protein, Rabkinesin-6, MKlp2

2 Images

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Immunoprecipitation - Anti-KIF20A antibody (AB70791)

HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70791 at 1 µg/ml for WB, subsequent to using for IP at 3 µg/mg lysate.
Lane 2 : IgG control.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-KIF20A antibody (ab70791)

Predicted band size: 100 kDa

false

Unknown

Western blot - Anti-KIF20A antibody (AB70791)

All lanes:

Western blot - Anti-KIF20A antibody (ab70791) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 100 kDa

Observed band size: 100 kDa,120 kDa,31 kDa,60 kDa,68 kDa,73 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human KIF20A. The exact immunogen used to generate this antibody is proprietary information.

O95235

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.

See full target information KIF20A

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 26:480 PubMed37745040

2023

Integrated bioinformatics analysis and experimental validation reveals hub genes of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Luo,Yumei Zhong,Yanding Guo,Jingwei Nie,Yimei Xu,Haiyan Zhou

PloS one 18:e0283490 PubMed37437070

2023

Histone methyltransferase SUV39H1 regulates the Golgi complex via the nuclear envelope-spanning LINC complex.

Applications

Unspecified application

Species

Unspecified reactive species

Miyu Nishino,Hiromasa Imaizumi,Yuhki Yokoyama,Jun Katahira,Hiroshi Kimura,Nariaki Matsuura,Miki Matsumura

Acta pharmaceutica Sinica. B 12:3861-3876 PubMed36213538

2022

Cucurbitacin B-induced G2/M cell cycle arrest of conjunctival melanoma cells mediated by GRP78-FOXM1-KIF20A pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlian Wei,Xin Chen,Yongyun Li,Ruoxi Li,Keting Bao,Liang Liao,Yuqing Xie,Tiannuo Yang,Jin Zhu,Fei Mao,Shuaishuai Ni,Renbing Jia,Xiaofang Xu,Jian Li

Frontiers in immunology 12:711145 PubMed34659201

2021

Development and Validation of a Combined Glycolysis and Immune Prognostic Model for Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Yaling Li,Ruiqun Qi,Lan Zhang

Scientific reports 11:5358 PubMed33686165

2021

The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal.

Applications

Unspecified application

Species

Unspecified reactive species

Miki Hieda,Taizo Matsumoto,Mari Isobe,Sadamu Kurono,Kaneko Yuka,Satoshi Kametaka,Jing-Ya Wang,Ya-Hui Chi,Kenji Kameda,Hiroshi Kimura,Nariaki Matsuura,Shuji Matsuura

Cancer management and research 12:13437-13449 PubMed33408516

2020

Identification of Core Genes Involved in the Metastasis of Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Peng,Yahui Wang,Likai Mao,Fang Fang,Han Guan

Cell cycle (Georgetown, Tex.) 19:2486-2495 PubMed32835591

2020

Long non-coding RNA UCA1 upregulates KIF20A expression to promote cell proliferation and invasion via sponging miR-204 in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qing He,Jianzhou Meng,Shuai Liu,Qiangcheng Zeng,Qinghua Zhu,Zhenlin Wei,Yibo Shao

Oncotarget 9:29727-29742 PubMed30038716

2018

MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERα and Survivin inhibition and enhances response to temozolomide.

Applications

Unspecified application

Species

Unspecified reactive species

George C Bobustuc,Amin B Kassam,Richard A Rovin,Sheila Jeudy,Joshua S Smith,Beth Isley,Maharaj Singh,Ameya Paranjpe,Kalkunte S Srivenugopal,Santhi D Konduri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIF20A antibody

Immunoprecipitation - Anti-KIF20A antibody (AB70791)

Western blot - Anti-KIF20A antibody (AB70791)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

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Primary Antibodies

Primary Antibodies

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