Anti-KIF21B antibody [EPR30126-519]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Immunohistochemical analysis of paraffin-embedded Mouse embryo 14.5 tissue labeling KIF21B with ab325182 at 1/1000 (0.519 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse embryo 14.5. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling KIF21B with ab325182 at 1/1000 (0.519 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on rat liver. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Immunohistochemical analysis of paraffin-embedded Rat embryo 14.5 tissue labeling KIF21B with ab325182 at 1/1000 (0.519 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat embryo 14.5. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Immunohistochemical analysis of paraffin-embedded Rat P3 brain tissue labeling KIF21B with ab325182 at 1/1000 (0.519 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat P3 brain. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling KIF21B with ab325182 at 1/1000 (0.519 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on mouse liver (PMID : 10225949). The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Immunohistochemical analysis of paraffin-embedded Mouse P3 brain tissue labeling KIF21B with ab325182 at 1/1000 (0.519 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse P3 brain. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver, lung(PMID : 29949770)

The bands beneath the target bands(200 kDa) are expected to be degradation products.

All lanes:

Western blot - Anti-KIF21B antibody [EPR30126-519] (ab325182) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 40 µg

Lane 2:

Human liver tissue lysate at 40 µg

Lane 3:

Human lung tissue lysate at 40 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 200 kDa,124 kDa

true

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : THP-1

In lanes 3-4, the lysates were stored at -80°C prior to Western Blotting. In lanes 1-2, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The bands beneath the target bands(200 kDa) are expected to be degradation products.

All lanes:

Western blot - Anti-KIF21B antibody [EPR30126-519] (ab325182) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) fresh whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) fresh whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) frozen whole cell lysate at 40 µg

Lane 4:

THP-1 (human monocytic leukemia monocyte) frozen whole cell lysate at 40 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver(PMID : 29949770)

The bands beneath the target bands(200 kDa) are expected to be degradation products.

To minimize protein degradation, lane 5 was lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Western blot - Anti-KIF21B antibody [EPR30126-519] (ab325182) at 1/1000 dilution

Lane 1:

Mouse E14 brain tissue lysate at 40 µg

Lane 2:

Mouse liver tissue lysate at 40 µg

Lane 3:

Rat E15 brain tissue lysate at 40 µg

Lane 4:

Rat liver tissue lysate at 40 µg

Lane 5:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 180s

• ELISA

Supplier Data

ELISA - Anti-KIF21B antibody [EPR30126-519] (AB325182)

Indirect ELISA analysis of ab325182 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human KIF21B; Human KIF21A.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with Human KIF21A.