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Anti-KIF22 antibody [EP2747Y] (ab75783) is a rabbit monoclonal antibody that is used to detect KIF22 in Western Blot. Suitable for Human samples.



- Specificity confirmed with KIF22 knockout cell line validation


Images

Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783), expandable thumbnail
  • Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783), expandable thumbnail

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Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed:25743205).

Alternative names

KID, KNSL4, KIF22, Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4

Recommended products

Anti-KIF22 antibody [EP2747Y] (ab75783) is a rabbit monoclonal antibody that is used to detect KIF22 in Western Blot. Suitable for Human samples.



- Specificity confirmed with KIF22 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2747Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 31st July 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783), expandable thumbnail

    Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)

    This image was generated using the hybridoma version of this product.

    Lanes 1 - 2: Merged signal (red and green). Green - ab75783 observed at 75 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab75783 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line Human KIF22 knockout HEK-293T cell line ab266817 (KIF22 knockout cell lysate Human KIF22 knockout HEK-293T cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75783 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: KIF22 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human KIF22 knockout HEK-293T cell line (Human KIF22 knockout HEK-293T cell line ab266817)

    Performed under reducing conditions.

    Predicted band size: 73 kDa

    Observed band size: 75 kDa

  • Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783), expandable thumbnail

    Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783)

    All lanes: Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

    Lane 2: 293T (Human embryonic kidney epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 75 kDa

    Exposure time: 40s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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