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AB75783

Anti-KIF22 antibody [EP2747Y]

5

(1 Review)

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(4 Publications)

Anti-KIF22 antibody [EP2747Y] (ab75783) is a rabbit monoclonal antibody detecting KIF22 in Western Blot. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2009

View Alternative Names

KID, KNSL4, KIF22, Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4

2 Images
Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)
  • WB

Supplier Data

Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)

All lanes:

Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate, prepared in 1% SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

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Exposure time: 40s

Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)
  • WB

Lab

Western blot - Anti-KIF22 antibody [EP2747Y] (AB75783)

This image was generated using the hybridoma version of this product.

Lanes 1 - 2 : Merged signal (red and green). Green - ab75783 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab75783 was shown to react with KIF22 in HEK-293T wild-type cells in western blot with loss of signal observed in KIF22 knockout cell line ab266817 (KIF22 knockout cell lysate ab258018). Wild-type and KIF22 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75783 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

KIF22 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human KIF22 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kif22-knockout-hek-293t-cell-line-ab266817'>ab266817</a>)

Predicted band size: 73 kDa

Observed band size: 75 kDa

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  • Carrier free

    Anti-KIF22 antibody [EP2747Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2747Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-KIF22 antibody [EP2747Y] (ab75783) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of KIF22?
Anti-KIF22 [EP2747Y] (ab75783) specifically detects a band for KIF22 (UniProt: Q14807) at a molecular weight of 73kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-KIF22 antibody [EP2747Y] (ab75783) has been confirmed by Western blot testing in KIF22 Knockout HEK-293 cells.

Other related products
We have a range of other formats of antibody clone [EP2747Y] also available for your convenience: ab75783, Carrier free - ab284718

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed : 25743205).
See full target information KIF22
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003086 PubMed40208872

2025

A nonenzymatic dependency on inositol-requiring enzyme 1 controls cancer cell cycle progression and tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Iratxe Zuazo-Gaztelu,David Lawrence,Ioanna Oikonomidi,Scot Marsters,Ximo Pechuan-Jorge,Catarina J Gaspar,David Kan,Ehud Segal,Kevin Clark,Maureen Beresini,Marie-Gabrielle Braun,Joachim Rudolph,Zora Modrusan,Meena Choi,Wendy Sandoval,Mike Reichelt,David C DeWitt,Pekka Kujala,Suzanne van Dijk,Judith Klumperman,Avi Ashkenazi

Nature 609:183-190 PubMed35922507

2022

A mitotic chromatin phase transition prevents perforation by microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian W G Schneider,Bryan A Gibson,Shotaro Otsuka,Maximilian F D Spicer,Mina Petrovic,Claudia Blaukopf,Christoph C H Langer,Paul Batty,Thejaswi Nagaraju,Lynda K Doolittle,Michael K Rosen,Daniel W Gerlich

Cell death discovery 8:208 PubMed35436989

2022

GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yeqing Ren,Biao Yang,Geng Guo,Jianping Zhang,Yanqi Sun,Dong Liu,Shihao Guo,Yongqiang Wu,Xiaogang Wang,Shule Wang,Wenju Zhang,Xiaolong Guo,Xuepeng Li,Ren Li,Jianhang He,Zihan Zhou

Nature communications 7:10597 PubMed26839278

2016

NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force.

Applications

IF

Species

Unspecified reactive species

Chenyu Li,Chenyi Xue,Qiaoyun Yang,Boon Chuan Low,Yih-Cherng Liou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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