AB122227

Anti-KIF4A/KIF4 antibody

Name: Anti-KIF4A/KIF4 antibody (ab122227) | Abcam
Brand: Abcam Limited
SKU: AB122227
Price: 480 USD
Availability: InStock

Be the first to review this product! Submit a review

(13 Publications)

Rabbit Polyclonal KIF4A/KIF4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KIF4A aa 950-1100.

View Alternative Names

KIF4, KIF4A, Chromosome-associated kinesin KIF4A, Chromokinesin-A

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (AB122227)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (AB122227)

ab122227, at a 1/300 dilution, staining KIF4A/KIF4 in paraffin embedded Human bone marrow tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB122227)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB122227)

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab122227.
Recommended conditions :
Fixation/Permeabilization : PFA/Triton X-100

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB122227)

ab122227, at 4 μg/ml, staining KIF4A/KIF4 in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (AB122227)

Immunohistochemical staining of Human bone marrow shows strong nuclear positivity in hematopoietic cells using ab122227

Unknown

Western blot - Anti-KIF4A/KIF4 antibody (AB122227)

All lanes:

Western blot - Anti-KIF4A/KIF4 antibody (ab122227) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 140 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KIF4A aa 950-1100. The exact immunogen used to generate this antibody is proprietary information.

O95239

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis (PubMed : 29848660). Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis (PubMed : 15297875, PubMed : 15625105). May play a role in mitotic chromosomal positioning and bipolar spindle stabilization (By similarity).

See full target information KIF4A

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology reports 51:385 PubMed38438773

2024

CDKN2A promoter methylation enhances self-renewal of glioblastoma stem cells and confers resistance to carmustine.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Yan-Feng Xi,Qi Zhao,Jiang-Hong Guo,Zhen Zhang,Mao-Bai Zhang,Jiang Chang,Yue-Qin Wu,Wen Su

The Journal of cell biology 222: PubMed37638884

2023

The abscission checkpoint senses chromatin bridges through Top2α recruitment to DNA knots.

Applications

Unspecified application

Species

Unspecified reactive species

Eleni Petsalaki,Sofia Balafouti,Athina A Kyriazi,George Zachos

Cancer medicine 12:12581-12592 PubMed37039264

2023

Knockdown of kinesin family member 4A inhibits cell proliferation, migration, and invasion while promoting apoptosis of urothelial bladder carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Maoyu Wang,Yidie Ying,Fang Meng,Hongliang Gao,Shuxiong Zeng,Yasheng Zhu,Anwei Liu,Zhensheng Zhang,Chuanliang Xu

Aging 13:24050-24070 PubMed34775374

2021

Identification of KIF4A as a prognostic biomarker for esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lingwei Wang,Gang Liu,Enkhbat Bolor-Erdene,Qinchuan Li,Yunqing Mei,Lei Zhou

BioMed research international 2021:8249293 PubMed34805404

2021

KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Cui-Cui Zhao,Fei-Ran Chen,Guo-Wei Feng,Fei Luo,Tao Jiang

PeerJ 9:e11455 PubMed34055488

2021

Identification of KIF4A as a pan-cancer diagnostic and prognostic biomarker via bioinformatics analysis and validation in osteosarcoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jiankang Pan,Xiaohua Lei,Xinzhan Mao

International journal of molecular medicine 47: PubMed33846765

2021

KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyi Zheng,Kaijie Wu,Zhongwei Gao,Huibing Li,Wensheng Li,Xiaohui Wang,Zhenguo Shi,Fei Xiao,Kaixuan Wang,Zhijun Li,Qingjiang Han

World journal of surgical oncology 18:315 PubMed33261630

2020

miR-29c-3p regulates proliferation and migration in ovarian cancer by targeting KIF4A.

Applications

Unspecified application

Species

Unspecified reactive species

Songwei Feng,Shanhui Luo,Chenchen Ji,Jia Shi

Clinical and investigative medicine. Medecine clinique et experimentale 43:E49-59 PubMed32971585

2020

KIF4A: A potential biomarker for prediction and prognostic of prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Chen,Maozhang Li,Shumin Fang,Xiaobo Zhou,Jinxian Liao,Shengbang Yang,Jianguo Zhu,Renqiang He,Jianming Lu,Funeng Jiang,Xiaoming Xu,Weide Zhong

Investigational new drugs 38:1730-1742 PubMed32533288

2020

The kinesin motor protein KIF4A as a potential therapeutic target in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guihong Liu,Yachun Lu,Liantao Li,Tao Jiang,Sufang Chu,Pingfu Hou,Jin Bai,Ming Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIF4A/KIF4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (AB122227)

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB122227)

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB122227)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (AB122227)

Western blot - Anti-KIF4A/KIF4 antibody (AB122227)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (13)

Product promise