AB3815

Anti-KIF4A/KIF4 antibody

4

(5 Reviews)

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(7 Publications)

Goat Polyclonal KIF4A/KIF4 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human KIF4A aa 1200-1250.

View Alternative Names

KIF4, KIF4A, Chromosome-associated kinesin KIF4A, Chromokinesin-A

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB3815)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (AB3815)

Immunocytochemical analysis of paraformaldehyde-fixed U-2 OS cells permeabilized with 0.15% Triton. Cells were incubated with ab3815 at 10 ug/ml for 1 hour followed by an Alexa Fluor^® 488 secondary at 4 ug/ml (green). The nuclear stain is DAPI (blue). Negative control is unimmunized goat IgG at 10 ug/ml, followed by an Alexa Fluor^® 488 secondary at 4 ug/ml (green).

Western blot - Anti-KIF4A/KIF4 antibody (AB3815)

WB

Supplier Data

Western blot - Anti-KIF4A/KIF4 antibody (AB3815)

All lanes:

Western blot - Anti-KIF4A/KIF4 antibody (ab3815) at 2 µg/mL

Lane 1:

NIH/3T3 nuclear lysate at 35 µg

Lane 2:

Human ovary lysate at 35 µg

Predicted band size: 140 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, ICC/IF

Immunogen

Synthetic Peptide within Human KIF4A aa 1200-1250. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis (PubMed : 29848660). Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis (PubMed : 15297875, PubMed : 15625105). May play a role in mitotic chromosomal positioning and bipolar spindle stabilization (By similarity).

See full target information KIF4A

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 30:427-440 PubMed30586323

2018

Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lysie Champion,Sumit Pawar,Naemi Luithle,Rosemarie Ungricht,Ulrike Kutay

Oncology research 24:455-461 PubMed28281966

2017

RNA Interference of IQ Motif Containing GTPase-Activating Protein 3 (IQGAP3) Inhibits Cell Proliferation and Invasion in Breast Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gaowu Hu,Ye Xu,Wenquan Chen,Jiandong Wang,Chunying Zhao,Ming Wang

Journal of cellular physiology 229:1466-74 PubMed24648201

2014

Core binding factor β (CBFβ) is retained in the midbody during cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Cesar Lopez-Camacho,Andre J van Wijnen,Jane B Lian,Janet L Stein,Gary S Stein

Molecular biology of the cell 23:2702-11 PubMed22621898

2012

Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis.

Applications

ICC/IF

Species

Human

Chi-Kuo Hu,Nurhan Ozlü,Margaret Coughlin,Judith J Steen,Timothy J Mitchison

Molecular biology of the cell 23:1024-34 PubMed22278743

2012

Midbody assembly and its regulation during cytokinesis.

Applications

ICC/IF

Species

Unspecified reactive species

Chi-Kuo Hu,Margaret Coughlin,Timothy J Mitchison

Current biology : CB 21:815-24 PubMed21565503

2011

KIF4 regulates midzone length during cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Kuo Hu,Margaret Coughlin,Christine M Field,Timothy J Mitchison

Molecular biology of the cell 21:380-92 PubMed19955215

2009

Mutual dependence of Mob1 and the chromosomal passenger complex for localization during mitosis.

Applications

Unspecified application

Species

Human

Lori Jo Wilmeth,Sanjay Shrestha,Gilbert Montaño,Jennifer Rashe,Charles Bradley Shuster

View all publications

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Product promise

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