AB124903

Anti-KIF4A/KIF4 antibody [EPR5459]

RabMAb
Recombinant
What is this?

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(13 Publications )

Rabbit Recombinant Monoclonal KIF4A/KIF4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 13 publications.

View Alternative Names

KIF4, KIF4A, Chromosome-associated kinesin KIF4A, Chromokinesin-A

3 Images

Flow Cytometry (Intracellular) - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

Intracellular flow cytometric analysis of permeablized 293 cells using ab124903 at 1/10 dilution (red) or a rabbit IgG negative control (green).

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Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

All lanes:

Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 140 kDa

Observed band size: 170 kDa

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OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

OI-RD Scanning

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OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-KIF4A/KIF4 antibody [EPR5459] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5459

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis (PubMed : 29848660). Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis (PubMed : 15297875, PubMed : 15625105). May play a role in mitotic chromosomal positioning and bipolar spindle stabilization (By similarity).

See full target information KIF4A

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature 609:183-190 PubMed35922507

2022

A mitotic chromatin phase transition prevents perforation by microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian W G Schneider,Bryan A Gibson,Shotaro Otsuka,Maximilian F D Spicer,Mina Petrovic,Claudia Blaukopf,Christoph C H Langer,Paul Batty,Thejaswi Nagaraju,Lynda K Doolittle,Michael K Rosen,Daniel W Gerlich

Experimental and therapeutic medicine 22:1339 PubMed34630693

2021

Enhanced expression of KIF4A in osteosarcoma predicts a poor prognosis and facilitates tumor growth by activation of the MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Zhu,Xiangfei Xu,Ming Zhang,Tong Wang

Medicine 100:e26130 PubMed34032761

2021

KIF4A as a novel prognostic biomarker in cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deng Yong Zhang,Shuo Shuo Ma,Wan-Liang Sun,Xue Chen Huang Lv,Zheng Lu

Journal of ovarian research 14:71 PubMed34034774

2021

Increased expression of KPNA2 predicts unfavorable prognosis in ovarian cancer patients, possibly by targeting KIF4A signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrong Cui,Honghong Wang,Xueqing Wu,Kai Huo,Xuan Jing

Molecular medicine reports 24: PubMed34013367

2021

KIF4A knockdown suppresses ovarian cancer cell proliferation and induces apoptosis by downregulating BUB1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wumin Jin,Lianmin Ye

Bioscience reports 41: PubMed33398330

2021

Identification of KIF4A and its effect on the progression of lung adenocarcinoma based on the bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yexun Song,Wenfang Tang,Hui Li

Thoracic cancer 12:512-524 PubMed33350074

2020

KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozheng Sun,Pengxiang Chen,Xue Chen,Wenjing Yang,Xuan Chen,Wei Zhou,Di Huang,Yufeng Cheng

Cell death & disease 11:508 PubMed32641713

2020

Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yang,Meixi Peng,Yixuan Hou,Yilu Qin,Xueying Wan,Pengpeng Zhu,Shuiqing Liu,Liping Yang,Huan Zeng,Ting Jin,Yuxiang Qiu,Qiao Li,Manran Liu

Clinical cancer research : an official journal of the American Association for Cancer Research 26:1516-1528 PubMed31796514

2019

Targeting the KIF4A/AR Axis to Reverse Endocrine Therapy Resistance in Castration-resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Cao,Zhengshuai Song,Hailong Ruan,Cheng Wang,Xiong Yang,Lin Bao,Keshan Wang,Gong Cheng,TianBo Xu,Wen Xiao,Zhiyong Xiong,Di Liu,Ming Yang,Diwei Zhou,Hongmei Yang,Ke Chen,Xiaoping Zhang

Cell death & disease 10:402 PubMed31127080

2019

Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Lan Wei,Wan-Xi Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIF4A/KIF4 antibody [EPR5459]

Flow Cytometry (Intracellular) - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (AB124903)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (13)

Product promise