Rabbit Polyclonal KIF5A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KIF5A aa 950 to C-terminus.
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Publications
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- Disease markers 2019:48249022019KIF5A Promotes Bladder Cancer Proliferation and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesDa-Wei Tian et. al.PubMed 31354888
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KIF5A aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12840
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.
Alternative names
NKHC1, KIF5A, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, Neuronal kinesin heavy chain, NKHC
Recommended products
Rabbit Polyclonal KIF5A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KIF5A aa 950 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KIF5A aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12840
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-KIF5A antibody (ab154414)
7.5% SDS PAGE
All lanes: Western blot - Anti-KIF5A antibody (ab154414) at 1/1000 dilution
All lanes: Mouse brain lysate at 50 µg
Predicted band size: 117 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab154414)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KIF5A using ab154414 at a 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5A antibody (ab154414)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Cal27 xenograft tissue, labeling KIF5A using ab154414 at a 1/100 dilution.
Western blot - Anti-KIF5A antibody (ab154414)
7.5% SDS PAGE
All lanes: Western blot - Anti-KIF5A antibody (ab154414) at 1/1000 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 117 kDa
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