Anti-KIF5A antibody [EPR29540-31]

11 product images

• 

  Western blot - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This antibody does not cross-react with mouse KIF5B or KIF5C.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-KIF5A antibody [EPR29540-31] (ab322900) at 1/1000 dilution

  Lane 1: 293T cells transfected with an empty vector containing a myc-his tag whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a mouse KIF5A expression vector containing a myc-his-tag, whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a mouse KIF5B expression vector containing a myc-his-tag, whole cell lysate at 20 µg

  Lane 4: 293T cells transfected with a mouse KIF5C expression vector containing a myc-his-tag, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 117 kDa, 36 kDa, 15 kDa

  Exposure time: 59s

• 

  Western blot - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: spleen(PMID 7514426).

  The identity of the bands lower than 100 kDa in lane 2 are unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-KIF5A antibody [EPR29540-31] (ab322900) at 1/1000 dilution

  Lane 1: Human cerebellum tissue lysate at 20 µg

  Lane 2: Human spleen tissue lysate at 20 µg

  Lane 3: Rat brain tissue lysate at 20 µg

  Lane 4: Rat cerebral cortex tissue lysate at 20 µg

  Lane 5: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 117 kDa, 36 kDa

  Exposure time: 180s

• 

  Western blot - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: spleen(PMID 7514426).

  Low expression: A20

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-KIF5A antibody [EPR29540-31] (ab322900) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse cerebral cortex tissue lysate at 20 µg

  Lane 3: Mouse cerebellum tissue lysate at 20 µg

  Lane 4: Mouse spleen tissue lysate at 20 µg

  Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 6: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 117 kDa, 36 kDa

  Exposure time: 180s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling KIF5A with ab322900 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on rat spleen.
  
  The section was incubated with ab322900 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling KIF5A with ab322900 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse spleen.
  
  The section was incubated with ab322900 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunohistochemical analysis of paraffin-embedded Rat cererbum tissue labeling KIF5A with ab322900 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat cerebrum.
  
  The section was incubated with ab322900 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunohistochemical analysis of paraffin-embedded Mouse cererbum tissue labeling KIF5A with ab322900 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse cerebrum (PMID: 37258573).
  
  The section was incubated with ab322900 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocyte cells labelling KIF5A with ab322900 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing no staining in mouse splenocyte (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling KIF5A with ab322900 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing no staining in mouse splenocyte (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling KIF5A with ab322900 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: ab322900 at a 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.

  -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at a 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody [EPR29540-31] (ab322900)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling KIF5A with ab322900 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  -ve control 1: ab322900 at a 1/50 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at a 1/1000 dilution.

  -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at a 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a 1/1000 dilution.