Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)

Rabbit Recombinant Monoclonal KIF5A antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (AB283661), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Not recommended	Tested
Mouse	Tested	Tested	Tested	Not recommended	Tested
Rat	Tested	Expected	Tested	Not recommended	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Target data

See full target information Kif5a

Function

Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL) (PubMed:12682084). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons (PubMed:21976701). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).

Additional Targets

Kif5b, Kif5c

Alternative names

Kiaa4086, Kif5, Nkhc1, Kif5a, Kinesin heavy chain isoform 5A, Kinesin heavy chain neuron-specific 1, Neuronal kinesin heavy chain, NKHC

Recommended products

Rabbit Recombinant Monoclonal KIF5A antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24325-98
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab283661 is the carrier-free version of Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
All lanes: Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 2: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg
Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg
Lane 4: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg
Lane 5: Rat brain cortex tissue lysate at 20 µg
Lane 6: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa
Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Degraded fragment (107 KD) has also been described (PMID: 11144355)
Exposure time: 10 seconds
All lanes: Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebral cortex tissue lysate at 20 µg
Lane 3: Mouse cerebellum tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat cerebellum tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 107 kDa, 117 kDa
Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Degraded fragment (107 KD) has also been described (PMID: 11144355)
Exposure time: 10 seconds
All lanes: Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Human hypothalamus tissue lysate at 20 µg
Secondary
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 117 kDa
Observed band size: 107 kDa, 117 kDa
Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 seconds
All lanes: Western blot - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049) at 1/5000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with KIF5A expression vector containing a myc-His-tag®, whole cell lysate at 2 µg
Lane 2: HEK-293T transfected with KIF5B expression vector containing a myc-His-tag®, whole cell lysate at 2 µg
Lane 3: HEK-293T transfected with KIF5C expression vector containing a myc-His-tag®, whole cell lysate at 2 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/50 (9.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/50 (9.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/50 (9.72 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution.
Immunoprecipitation - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
KIF5A + KIF5B + KIF5C was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
All lanes: Immunoprecipitation - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049)
Observed band size: 117 kDa
Flow Cytometry (Intracellular) - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
KIF5A + KIF5B + KIF5C was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug
Lane 2: Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
All lanes: Immunoprecipitation - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049)
Observed band size: 117 kDa
Flow Cytometry (Intracellular) - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] - BSA and Azide free (ab283661)
This data was developed using Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] ab271049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.