Anti-KIF5B antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (AB5629)

ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37°C were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1 : 2000). Images were taken on wide-field fluorescent microscope at 63X magnification.

• WB

Supplier Data

Western blot - Anti-KIF5B antibody (AB5629)

All lanes:

Western blot - Anti-KIF5B antibody (ab5629) at 1/2000 dilution

Lane 1:

Mouse brain Whole cell extract at 40 µg

Lane 2:

Mouse lung Whole cell extract at 40 µg

Lane 3:

Rat brain Whole cell extract at 40 µg

Lane 4:

Rat liver Whole cell extract at 40 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L),HRP at 1/4000 dilution

Predicted band size: 110 kDa

false

• WB

Supplier Data

Western blot - Anti-KIF5B antibody (AB5629)

All lanes:

Western blot - Anti-KIF5B antibody (ab5629) at 1/4000 dilution

Lane 1:

Mouse Cerebellum whole cell extract at 30 µg

Lane 2:

Rat Brain whole cell extract at 30 µg

Lane 3:

Mouse Brain whole cell extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

Predicted band size: 110 kDa

false