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AB5629

Anti-KIF5B antibody

5

(2 Reviews)

|

(20 Publications)

Rabbit Polyclonal KIF5B antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human KIF5B aa 350-400.

View Alternative Names

KNS, KNS1, KIF5B, Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (AB5629)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (AB5629)

ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37°C were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1 : 2000). Images were taken on wide-field fluorescent microscope at 63X magnification.

Western blot - Anti-KIF5B antibody (AB5629)
  • WB

Supplier Data

Western blot - Anti-KIF5B antibody (AB5629)

All lanes:

Western blot - Anti-KIF5B antibody (ab5629) at 1/4000 dilution

Lane 1:

Mouse Cerebellum whole cell extract at 30 µg

Lane 2:

Rat Brain whole cell extract at 30 µg

Lane 3:

Mouse Brain whole cell extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

Predicted band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KIF5B aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P33176

Specificity

This antibody is specific for KIF5B and does not detect other kinesin isotypes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/6000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/6000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed : 20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed : 24088571).
See full target information KIF5B
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:114 PubMed40328804

2025

Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Yang,Zhuoran Ma,Piaopiao Lian,Yi Wu,Ke Liu,Zhaoyuan Zhang,Zhicheng Tang,Yan Xu,Xuebing Cao

Nature communications 15:10829 PubMed40016183

2025

Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Hei Li,Noortje Kersten,Nazmiye Özkan,Dan T M Nguyen,Max Koppers,Harm Post,Maarten Altelaar,Ginny G Farias

The Journal of cell biology 222: PubMed37213076

2023

Inhibition of endolysosome fusion increases exosome secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesh Vilas Shelke,Chad D Williamson,Michal Jarnik,Juan S Bonifacino

eLife 12: PubMed36897066

2023

Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Marcelo Lazo,Giampietro Schiavo

BMC molecular and cell biology 23:26 PubMed35794526

2022

Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Wei Lee,Chia-Chen Kuo,Chi-Jung Liang,Huei-Jyuan Pan,Chia-Ning Shen,Chau-Hwang Lee

Nature 599:662-666 PubMed34789877

2021

Herpesviruses assimilate kinesin to produce motorized viral particles.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin E Pegg,Sofia V Zaichick,Ewa Bomba-Warczak,Vladimir Jovasevic,DongHo Kim,Himanshu Kharkwal,Duncan W Wilson,Derek Walsh,Patricia J Sollars,Gary E Pickard,Jeffrey N Savas,Gregory A Smith

International journal of molecular sciences 22: PubMed33466445

2021

Intermittent Hypoxic Conditioning Rescues Cognition and Mitochondrial Bioenergetic Profile in the Triple Transgenic Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sónia C Correia,Nuno J Machado,Marco G Alves,Pedro F Oliveira,Paula I Moreira

Journal of cell science 133: PubMed32661088

2020

Microtubule motor transport in the delivery of melanosomes to the actin-rich apical domain of the retinal pigment epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Jiang,Antonio E Paniagua,Stefanie Volland,Hongxing Wang,Adarsh Balaji,David G Li,Vanda S Lopes,Barry L Burgess,David S Williams

The Journal of cell biology 219: PubMed31910261

2020

Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain.

Applications

Unspecified application

Species

Unspecified reactive species

Min Lee,Yen-Chen Liu,Chen Chen,Chi-Huan Lu,Shao-Tzu Lu,Tzyy-Nan Huang,Meng-Tsung Hsu,Yi-Ping Hsueh,Pei-Lin Cheng

Laboratory investigation; a journal of technical m 99:1822-1834 PubMed31409893

2019

Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Nardi,Philip Fitchev,Kyrsten M Brooks,Omar E Franco,Kevin Cheng,Simon W Hayward,Michael A Welte,Susan E Crawford
View all publications
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Product promise

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