Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (AB248540)

This data was developed using ab133537, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling KIFAP3 with ab133537 at 1/50

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (AB248540)

This data was developed using ab133537, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-KIFAP3 antibody [EPR5600] (<a href='/en-us/products/primary-antibodies/kifap3-antibody-epr5600-ab133537'>ab133537</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 91 kDa

Observed band size: 100 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-KIFAP3 antibody [EPR5600] - BSA and Azide free (AB248540)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.