Rabbit Polyclonal Kindlin 2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 14 publications.
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Publications
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- PloS one 17:e02787742022Alzheimer's risk factor FERMT2 promotes the progression of colorectal carcinoma via Wnt/β-catenin signaling pathway and contributes to the negative correlation between Alzheimer and cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenzhen Xia,Zhaoyu Gao,Xia Jiang,Lei Jiang,Yushi Qin,Di Zhang,Pei Tian,Wanchang Wang,Qi Zhang,Rui Zhang,Nan Zhang,Shunjiang XuPubMed 36480537
- Cell cycle (Georgetown, Tex.) 20:2413-24272021YBX1 knockdown induces renal cell carcinoma cell apoptosis via Kindlin-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiqi Cui,Chao Wang,Shuang Liu,Runxuan Du,Shaoping Tian,Ruibing Chen,Hua Geng,Saravanan Subramanian,Yuanjie Niu,Yong Wang,Dan YuePubMed 34709966
- Frontiers in neuroscience 14:5911382021MicroRNA-138 Overexpression Alters Aβ42 Levels and Behavior in Wildtype Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmanuelle Boscher,Claudia Goupil,Serena Petry,Rémi Keraudren,Andréanne Loiselle,Emmanuel Planel,Sébastien S HébertPubMed 33519353
- Aging 11:6273-62852019Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan-Yuan Wei et. al.PubMed 31427543
- Journal of Alzheimer's disease : JAD 68:1243-12552019Copy Number Variants in miR-138 as a Potential Risk Factor for Early-Onset Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmanuelle Boscher,Thomas Husson,Olivier Quenez,Annie Laquerrière,Florent Marguet,Kevin Cassinari,David Wallon,Olivier Martinaud,Camille Charbonnier,Gaël Nicolas,Jean-François Deleuze,Anne Boland,Mark Lathrop,Thierry Frébourg,Dominique Campion,Sébastien S Hébert,Anne Rovelet-LecruxPubMed 30909216
- Cancer cell 35:46-63.e102018p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanagiotis Karras,Erica Riveiro-Falkenbach,Estela Cañón,Cristina Tejedo,Tonantzin G Calvo,Raúl Martínez-Herranz,Direna Alonso-Curbelo,Metehan Cifdaloz,Eva Perez-Guijarro,Gonzalo Gómez-López,Pilar Ximenez-Embun,Javier Muñoz,Diego Megias,David Olmeda,Jorge Moscat,Pablo L Ortiz-Romero,Jose L Rodríguez-Peralto,María S SoengasPubMed 30581152
- Cancer cell international 18:1662018Prognostic value of Kindlin-2 expression in patients with solid tumors: a meta-analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng Liu et. al.PubMed 30386175
- Nature biotechnology :2018On-demand manufacturing of clinical-quality biopharmaceuticals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura E Crowell et. al.PubMed 30272677
- The Journal of cell biology 217:1929-19402018Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthias Spiess et. al.PubMed 29632027
- Nature communications 6:75312015Kindlin-2 controls TGF-β signalling and Sox9 expression to regulate chondrogenesis.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
Alternative names
Fermitin family homolog 2, Kindlin-2, Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, MIG-2, PH domain-containing family C member 1, MIG2, KIND2, PLEKHC1, FERMT2
Recommended products
Rabbit Polyclonal Kindlin 2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 14 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kindlin-2 also known as FERMT2 is a mechanistic component involved in cellular adhesion processes. This protein has a molecular weight of approximately 75 kDa. Kindlin-2 is found in various tissues with high expression in epithelial cells and muscle tissues. It works together with integrins to maintain cell adhesion to the extracellular matrix performing an important role in cell communication with its surroundings.
- Biological function summary
Kindlin-2 contributes to integrin activation and cytoskeletal organization by forming part of a larger complex. This complex stabilizes integrin-talin interactions enhancing the affinity of integrins for their ligands. This function of Kindlin-2 aids in dynamically regulating cell shape migration and mechanical signaling. Its role is significant for normal cellular function and homeostasis because it helps cells to attach and detach when necessary which is important for cell movement and tissue repair.
- Pathways
The action of Kindlin-2 integrates with key signaling pathways like the Wnt and PI3K/AKT pathways. In these pathways Kindlin-2 assists in transmitting signals necessary for cellular proliferation and survival. It interacts with other proteins such as talin and integrin β subunits forming a bridge between the cytoskeleton and external signals. This interaction allows cells to adapt to changes in their environment by modulating cell adhesion and migration.
- Associated diseases and disorders
Kindlin-2's function relates to conditions such as cancer and fibrosis. In cancer Kindlin-2 aids tumor cell migration and metastasis linking it to the pathological spread of cancerous cells. In fibrosis Kindlin-2 influences the excessive deposition of extracellular matrix components contributing to tissue scarring. The protein also interacts with fibronectin and collagens in these contexts showing its involvement in extracellular matrix remodeling and pathological tissue changes.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-Kindlin 2 antibody (ab74030)
Lanes 1-3: Merged signal (red and green). Green - ab74030 observed at 78 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab74030 Anti-Kindlin 2 antibody was shown to specifically react with Kindlin 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FERMT2 (Kindlin 2) knockout HeLa cell line ab265247 (knockout cell lysate Human FERMT2 (Kindlin 2) knockout HeLa cell lysate ab257436) was used. Wild-type and Kindlin 2 knockout samples were subjected to SDS-PAGE. ab74030 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Kindlin 2 antibody (ab74030) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FERMT2 knockout HeLa cell lysate at 20 µg
Lane 3: Saos-2 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
This image is courtesy of a customer review submitted by Ana Kasirer-Friede,UCSD , United StatesWestern blot - Anti-Kindlin 2 antibody (ab74030)
The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
All lanes: Western blot - Anti-Kindlin 2 antibody (ab74030)
Predicted band size: 78 kDa
Immunoprecipitation - Anti-Kindlin 2 antibody (ab74030)
Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 78kDa; Kindlin 2All lanes: Immunoprecipitation - Anti-Kindlin 2 antibody (ab74030)
Predicted band size: 78 kDa
Western blot - Anti-Kindlin 2 antibody (ab74030)
All lanes: Western blot - Anti-Kindlin 2 antibody (ab74030) at 1 µg/mL
Lane 1: HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg
Lane 2: SAOS-2 (sarcoma osteogenic) Whole Cell lysate at 10 µg
Lane 3: Human testis tissue lysate - total protein (ab30257) at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 8min
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