Rabbit Polyclonal Kinectin 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KTN1 aa 150-350.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KTN1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q86UP2
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.
Alternative names
CG1, KIAA0004, KTN1, Kinectin, CG-1 antigen, Kinesin receptor
Recommended products
Rabbit Polyclonal Kinectin 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KTN1 aa 150-350.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KTN1 aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q86UP2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kinectin 1 also known as kinectin is a protein with a molecular mass of approximately 156 kDa. Predominantly located in the endoplasmic reticulum kinectin 1 functions as a membrane anchor for kinesin motors. This protein facilitates the movement of organelles along microtubules by binding to kinesin which is essential for intracellular transport. Kinectin 1 expression can be detected in various tissues but is notably high in the brain liver and kidney reflecting its involvement in critical cellular processes.
- Biological function summary
Kinectin 1 plays an important role in the organization of the cytoskeleton. It interacts with other cytoskeletal components and participates in the assembly of the Golgi apparatus. Kinectin 1 also forms part of the larger motor protein complex that includes kinesin light chains and other adaptors critical for vesicle-mediated transport. This complex is responsible for maintaining cellular homeostasis and efficient distribution of materials within the cell.
- Pathways
Kinectin 1 is integral to the intracellular trafficking and signaling pathways. It is closely associated with the secretory and endocytic pathways both of which depend on accurate and timely transport of proteins and membranes. Kinectin 1 collaborates with proteins like kinesin and cytoplasmic dynein facilitating directional transport along microtubules. This interaction is vital for the proper functioning of cellular logistics and signaling cascades.
- Associated diseases and disorders
Research shows that kinectin 1 is connected to neurodegenerative diseases such as Alzheimer's. Disruption in its normal function or expression can lead to impaired intracellular transport contributing to the pathogenesis of such conditions. Additionally kinectin 1 interacts with kinesin motor proteins in processes that when dysregulated may relate to metabolic disorders impacting cellular energy distribution. Understanding these connections could inform therapeutic strategies for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinectin 1 antibody (ab224324)
Paraffin-embedded human cerebral cortex tissue stained for Kinectin 1 using ab224324 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-Kinectin 1 antibody (ab224324)
All lanes: Western blot - Anti-Kinectin 1 antibody (ab224324) at 1/100 dilution
All lanes: RT4 (human urinary bladder cancer cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 156 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Kinectin 1 antibody (ab224324)
PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Kinectin 1 (green) using ab224324 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinectin 1 antibody (ab224324)
Paraffin-embedded human testis tissue stained for Kinectin 1 using ab224324 at 1/50 dilution in immunohistochemical analysis.
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