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AB154868

Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal KIF2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIF2, KNS2, KIF2A, Kinesin-like protein KIF2A, Kinesin-2, hK2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)

Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma labeling Kinesin Heavy Chain 2 with ab154868 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)
  • WB

Lab

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

Predicted band size: 187 kDa

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Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)
  • WB

Unknown

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (AB154868)

All lanes:

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 187 kDa

false

  • Carrier free

    Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10740(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kinesin Heavy Chain 2 also known as kinesin-2 or KIF3A is a motor protein involved in intracellular transport. It has a molecular mass of about 83 kDa. Kinesin-2 functions by moving along microtubules converting chemical energy from ATP into mechanical work to transport cellular cargo. This protein primarily operates in cellular regions with high microtubule activity such as neurons and epithelial cells. Kinesin-2 is vital for ciliary function as it transports essential components along the axoneme of motile and primary cilia.
Biological function summary

Kinesin-2 operates as part of the kinesin-2 family complex which also includes KIF3B and a kinesin-associated protein (KAP). It manages the anterograde transport of proteins and vesicles necessary for ciliary growth and function. This system is essential for maintaining cilia which play roles in cell signaling and locomotion. Without the proper action of kinesin-2 ciliary assembly and maintenance become impaired leading to compromised cell function.

Pathways

Kinesin-2 is critical in the intraflagellar transport (IFT) pathway and the Hedgehog signaling pathway. In the IFT pathway kinesin-2 works closely with the IFT proteins to shuttle cargo necessary for cilia function from the cell body to the cilia tip. In the Hedgehog pathway kinesin-2 helps transport signaling molecules to cilia which is important for pathway activation. This protein interacts with the IFT-B complex ensuring efficient signal transduction necessary for correct cellular responses.

Kinesin-2 malfunctions associate with ciliopathies and certain types of cancer. Defective kinesin-2 function can cause ciliopathies like Bardet-Biedl syndrome due to its role in ciliary transport. Additionally disruption of kinesin-2-related transport in Hedgehog signaling links to basal cell carcinoma where this signaling pathway often becomes aberrant. These conditions emphasize the importance of kinesin-2 in proper cellular function and its contributions to human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
See full target information KIF2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:611 PubMed37400785

2023

Upregulation of hsa_circ_0002003 promotes hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Zhou,Qianwen Wang,Jun Hou,Xiangwei Wu,Lianghai Wang,Xueling Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com