Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] - BSA and Azide free (AB249167)

This data was developed using ab154868, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100.

• WB

Lab

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] - BSA and Azide free (AB249167)

This data was developed using ab154868, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 μg)

Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (<a href='/en-us/products/primary-antibodies/kinesin-heavy-chain-2-antibody-epr10740b-ab154868'>ab154868</a>)

Predicted band size: 187 kDa

false

• WB

Unknown

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] - BSA and Azide free (AB249167)

This data was developed using ab154868, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] - BSA and Azide free (ab249167) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 187 kDa

false