JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB85996

Anti-Kinetochore antibody

1

(1 Review)

|

(2 Publications)

Rabbit Polyclonal Kinetochore antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KNTC1 aa 350-450.

View Alternative Names

KIAA0166, ROD, KNTC1, Kinetochore-associated protein 1, Rough deal homolog, HsROD, Rod, hRod

2 Images
Immunoprecipitation - Anti-Kinetochore antibody (AB85996)
  • IP

Unknown

Immunoprecipitation - Anti-Kinetochore antibody (AB85996)

Detection of Kinetochore by Western Blot of Immunprecipitate.
ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Kinetochore antibody (ab85996)

Predicted band size: 250 kDa

false

Western blot - Anti-Kinetochore antibody (AB85996)
  • WB

Unknown

Western blot - Anti-Kinetochore antibody (AB85996)

All lanes:

Western blot - Anti-Kinetochore antibody (ab85996) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 250 kDa

Observed band size: 115 kDa,175 kDa,250 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human KNTC1 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P50748

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab85996 was affinity purified using an epitope specific to Kinetochore immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kinetochore is a protein complex that plays an important role during cell division particularly in ensuring proper chromosome segregation. It attaches to the centromere of each chromosome and serves as the anchor point for spindle microtubules. This interaction is essential for the movement and alignment of chromosomes on the metaphase plate. Although its exact molecular mass can vary due to its complex nature kinetochore is typically composed of multiple proteins including CENP proteins. It is found within the eukaryotic cells specifically expressed during mitosis and meiosis.
Biological function summary

Kinetochore is integral in forming a protein structure known as the kinetochore complex which is also called the spindle assembly checkpoint complex. It ensures chromosomes are properly aligned before the cell progresses to anaphase. Kinetochore also signals to delay anaphase until all chromosomes are correctly bi-oriented. Within this complex kinetochore proteins work together with regulatory proteins to prevent chromosome missegregation.

Pathways

Kinetochore plays an essential role in cell cycle regulation and mitotic spindle checkpoint pathways. It interacts closely with Aurora B and MAD2 proteins which both regulate the progression through mitosis. The mitotic spindle checkpoint pathway ensures chromosomes do not prematurely separate therefore maintaining genomic stability.

Kinetochore has a connection to conditions related to aneuploidy and cancer. Defective kinetochore function can lead to chromosome missegregation contributing to aneuploidy a feature observed in various cancers. Furthermore kinetochore malfunction is linked to Alzheimer's disease through its interaction with the tau protein which plays a role in neurodegenerative processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores (PubMed : 11146660, PubMed : 11590237, PubMed : 15824131). Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.
See full target information KNTC1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 11:12 PubMed30880332

2019

Overexpression of Dlx2 enhances osteogenic differentiation of BMSCs and MC3T3-E1 cells via direct upregulation of Osteocalcin and Alp.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfei Zhang,Wenbin Zhang,Jiewen Dai,Xudong Wang,Steve Guofang Shen

International journal of oncology 54:1053-1060 PubMed30628654

2019

shRNA‑mediated knockdown of KNTC1 suppresses cell viability and induces apoptosis in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Tao Liu,Li Min,Yong-Jun Wang,Peng Li,Yong-Dong Wu,Shu-Tian Zhang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB254630

Anti-Kinetochore antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinetochore antibody (AB254630)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB109496

Anti-HEC1/HEC antibody [EPR5342]

RabMAb|Recombinant

Western blot - Anti-HEC1/HEC antibody [EPR5342] (AB109496)

  • 0
  • 0
Primary Antibodies

AB45694

Anti-CENPA antibody [EP800Y] - Centromere Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

  • 1
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB133583

Anti-CENPE antibody [EPR4542(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-CENPE antibody [EPR4542(2)] (AB133583)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com