AB97761

Anti-Kininogen 1 antibody

Name: Anti-Kininogen 1 antibody (ab97761) | Abcam
Brand: Abcam Limited
SKU: AB97761
Rating: 3 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Kininogen antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KNG1 aa 1-450.

View Alternative Names

BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody (AB97761)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody (AB97761)

ab97761 at 1/500 dilution staining Kininogen in paraffin-embedded A549 xenograft tissue by immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-Kininogen 1 antibody (AB97761)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kininogen 1 antibody (AB97761)

ab76197 at 1/200 dilution staining Kininogen in paraformaldehyde-fixed HeLa cells by immunofluorescence .
Lower image is merged with DNA probe.

Unknown

Western blot - Anti-Kininogen 1 antibody (AB97761)

10% SDS-PAGE.

All lanes:

Western blot - Anti-Kininogen 1 antibody (ab97761) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KNG1 aa 1-450. The exact immunogen used to generate this antibody is proprietary information.

P01042

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kininogen 1 often referred to as kininogen-1 or high molecular weight kininogen (HMWK) is a significant protein in the body with a mass of approximately 120 kDa. It is primarily expressed in the liver and secreted into plasma. Kininogen 1 acts as a precursor for kinins vital for various physiological processes. The cleavage of kininogen releases bradykinin a potent vasodilator which suggests roles in blood pressure regulation and inflammation.

Biological function summary

Kininogen 1 influences processes such as blood coagulation and inflammation. It is a part of the kallikrein-kinin system where it helps in generating kallidin and bradykinin. These peptides play important roles in vasodilation increasing vascular permeability and pain mechanisms. Kininogen 1 serves as a cofactor for the activation of coagulation factor XII linking coagulation and inflammation pathways.

Pathways

Kininogen 1 plays a critical role in the kallikrein-kinin and contact activation pathways. Within the kallikrein-kinin pathway it works along with the kallikrein enzyme to produce kinins. In the contact activation pathway it associates with prekallikrein and factor XII influencing both coagulation and inflammatory responses in the body.

Kininogen 1 is associated with hereditary angioedema and cardiovascular disease. Its role in bradykinin generation relates to the excessive swelling seen in hereditary angioedema due to bradykinin buildup. Additionally its interactions with coagulation factors such as factor XII connect it to cardiovascular disorders where improper coagulation regulation can lead to pathological blood clotting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).

See full target information KNG1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 29:691-711 PubMed36550594

2022

LncRNA LINC01018/miR-942-5p/KNG1 axis regulates the malignant development of glioma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfang Xu,Jianli Wang,Mingfei Zhao,Chenguang Li,Shen Hong,Jianmin Zhang

Journal of molecular endocrinology 59:105-119 PubMed28539318

2017

Serum estradiol levels in controlled ovarian stimulation directly affect the endometrium.

Applications

IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Kamran Ullah,Tanzil Ur Rahman,Hai-Tao Pan,Meng-Xi Guo,Xin-Yan Dong,Juan Liu,Lu-Yang Jin,Yi Cheng,Zhang-Hong Ke,Jun Ren,Xian-Hua Lin,Xiao-Xiao Qiu,Ting-Ting Wang,He-Feng Huang,Jian-Zhong Sheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kininogen 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody (AB97761)

Immunocytochemistry/ Immunofluorescence - Anti-Kininogen 1 antibody (AB97761)

Western blot - Anti-Kininogen 1 antibody (AB97761)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise