AB124737

Anti-Kininogen 1 antibody [EPR6097]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal Kininogen antibody. Suitable for IHC-P, IP, ELISA, WB and reacts with Human, Recombinant fragment samples. Cited in 5 publications.

View Alternative Names

BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human kidney tissues by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ELISA - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

ELISA

Unknown

ELISA - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

ELISA analysis of Human Kininogen-1 recombinant protein at 1000 ng/mL with ab124737. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Unknown

Western blot - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

ab124737 is detecting three isoforms of Kininogen 1 : full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).

All lanes:

Western blot - Anti-Kininogen 1 antibody [EPR6097] (ab124737) at 1/10000 dilution

Lane 1:

Human plasma at 10 µg

Lane 2:

Human platelet tissue lysate at 10 µg

Lane 3:

Human ovarian cancer tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 71 kDa

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OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

CiteAb

Western blot - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

Kininogen 1 western blot using anti-Kininogen 1 antibody [EPR6097] ab124737. Publication image and figure legend from Schrödter, S., Braun, M., et al., 2016, Mol Cancer, PubMed 26831905.

ab124737 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124737 please see the product overview.

Western blot experiments were performed to determine the protein expression in eight corresponding normal (N) and clear cell renal cell carcinoma (T) tissue. NPTX2 was increased in tumor tissue, whereas FXYD4, KNG1, SLC12A1 and SLC6A3 were decreased in tumors. NDUF4AL2 levels were similar, but the bands were of different size in tumor and normal samples

false

Carrier free

Anti-Kininogen antibody [EPR6097] - BSA and Azide free (Capture)
Carrier free

Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6097

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kininogen 1 often referred to as kininogen-1 or high molecular weight kininogen (HMWK) is a significant protein in the body with a mass of approximately 120 kDa. It is primarily expressed in the liver and secreted into plasma. Kininogen 1 acts as a precursor for kinins vital for various physiological processes. The cleavage of kininogen releases bradykinin a potent vasodilator which suggests roles in blood pressure regulation and inflammation.

Biological function summary

Kininogen 1 influences processes such as blood coagulation and inflammation. It is a part of the kallikrein-kinin system where it helps in generating kallidin and bradykinin. These peptides play important roles in vasodilation increasing vascular permeability and pain mechanisms. Kininogen 1 serves as a cofactor for the activation of coagulation factor XII linking coagulation and inflammation pathways.

Pathways

Kininogen 1 plays a critical role in the kallikrein-kinin and contact activation pathways. Within the kallikrein-kinin pathway it works along with the kallikrein enzyme to produce kinins. In the contact activation pathway it associates with prekallikrein and factor XII influencing both coagulation and inflammatory responses in the body.

Kininogen 1 is associated with hereditary angioedema and cardiovascular disease. Its role in bradykinin generation relates to the excessive swelling seen in hereditary angioedema due to bradykinin buildup. Additionally its interactions with coagulation factors such as factor XII connect it to cardiovascular disorders where improper coagulation regulation can lead to pathological blood clotting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).

See full target information KNG1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1545780 PubMed40376586

2025

Inhibiting ADORA1 enhances glioma apoptosis and increases its sensitivity to anti-PD1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Jiang Li,Zhi-Yun Yu,Hua-Ping Gao,Yi-Ran Xu,Xue-Yuan Li,Wei Jiang,Di Chen,Dong-Ming Yan,Chao Yang,Xian-Zhi Liu

British journal of haematology 205:1963-1973 PubMed39228027

2024

Association of biomarkers of endothelial function, coagulation activation and kidney injury with persistent albuminuria in sickle cell anaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Laila Elsherif,Yihan Tang,Kammie L Patillo,David Wichlan,Ugochi O Ogu,Kristina Landes,Paula McCune,Lara C Scott,Whitney Gulledge,Woodi H Woodland,Marquita Nelson,Laura R Loehr,Robert M Cronin,Payal C Desai,Laura Y Zhou,David M Pollock,Fei Zou,Jianwen Cai,Vimal K Derebail,Kenneth I Ataga

Journal of Cancer 9:540-547 PubMed29535795

2018

Identification of Kininogen 1 as a Serum Protein Marker of Colorectal Adenoma in Patients with a Family History of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiekai Yu,Yanqin Huang,Chen Lin,Xiaofen Li,Xuefeng Fang,Chenhan Zhong,Ying Yuan,Shu Zheng

Molecular cancer 15:10 PubMed26831905

2016

Identification of the dopamine transporter SLC6A3 as a biomarker for patients with renal cell carcinoma.

Applications

Species

Human

Sarah Schrödter,Martin Braun,Isabella Syring,Niklas Klümper,Mario Deng,Doris Schmidt,Sven Perner,Stefan C Müller,Jörg Ellinger

Expert review of proteomics 12:97-107 PubMed25434962

2014

Screening and identification of potential predictive biomarkers for Down's syndrome from second trimester maternal serum.

Applications

Species

Human

Yuan Yao,Ying Jiang,Mei Han,Yu Xia,Yuchen He,Yonghu Wang,Yang Luo,Bo Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kininogen 1 antibody [EPR6097]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

ELISA - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

Western blot - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

Western blot - Anti-Kininogen 1 antibody [EPR6097] (AB124737)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise