AB65796

Anti-Kir2.1/KCNJ2 antibody

Name: Anti-Kir2.1/KCNJ2 antibody (ab65796) | Abcam
Brand: Abcam Limited
SKU: AB65796
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(18 Publications)

Rabbit Polyclonal Kir2.1/KCNJ2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 18 publications.

View Alternative Names

IRK1, KCNJ2, Inward rectifier potassium channel 2, Cardiac inward rectifier potassium channel, Inward rectifier K(+) channel Kir2.1, IRK-1, hIRK1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody (AB65796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody (AB65796)

ab65796 staining Kir2.1/KCNJ2 in human colon.
Left panel : with primary antibody at 1 : 1000 dilution. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody (AB65796)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody (AB65796)

ICC/IF image of ab65796 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65796, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Kir2.1 protein also known as KCNJ2 belongs to a family of inward-rectifier potassium channels. Kir2.1 is important for setting and stabilizing the resting membrane potential. It has a molecular mass of approximately 48 kDa. Kir2.1 is mainly expressed in the heart and brain tissues and it facilitates the flow of potassium ions across the cell membrane with high efficiency. This modulation of potassium ion movement is critical for maintaining membrane potential and is vital for the normal electrical activity in excitable tissues.

Biological function summary

This process aids in the regulation of cellular excitability. Kir2.1 is an integral part of cell membranes and often associates with other proteins to form a functional channel complex. This complex plays a role in neuronal signaling and cardiac rhythm regulation contributing to various physiological functions in muscle and nerve tissue. Its roles underpin how potassium currents modulate nerve impulses and heartbeats reflecting its participation in the body's complex communication and control systems.

Pathways

The Kir2.1 channel influences the cardiac action potential and is involved in maintaining the stability of the heart’s electrical signaling. It is part of the cardiac conduction pathway. The channel interacts with other cardiac proteins like KCNJ3 and KCNJ5 complementing their function in the repolarization phase of the cardiac action potential. Kir2.1's presence in pathways responsible for repolarization highlights the precision needed for effective cardiac and neurological functions.

Mutations in the Kir2.1 gene KCNJ2 relate to Andersen-Tawil syndrome and cardiac arrhythmias. Andersen-Tawil syndrome characterized by periodic paralysis cardiac arrhythmias and developmental anomalies highlights the multifaceted role Kir2.1 plays in cellular function. Kir2.1 mutations disrupt electrical stability in heart and skeletal muscle tissues. Similar mutations associate with connections to other membrane proteins like KCNH2 often exacerbating arrhythmic risk a critical factor in heart-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it (PubMed : 36149965, PubMed : 7590287, PubMed : 9490857). Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages (PubMed : 7590287, PubMed : 7696590). The inward rectification is mainly due to the blockage of outward current by internal magnesium (PubMed : 9490857). Can be blocked by extracellular barium or cesium (PubMed : 7590287, PubMed : 7696590). Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues (PubMed : 7590287, PubMed : 7696590, PubMed : 7840300).

See full target information KCNJ2

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39151955

2024

Proper Frequency of Perinatal Retinal Waves Is Essential for the Precise Wiring of Visual Axons in Nonimage-Forming Nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago Negueruela,Cruz Morenilla-Palao,Salvador Sala,Patricia Ordoño,Macarena Herrera,Yaiza Coca,Maria Teresa López-Cascales,Danny Florez-Paz,Ana Gomis,Eloísa Herrera

Communications biology 6:1236 PubMed38062109

2023

CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Julie L Han,Yuli W Heinson,Christianne J Chua,Wei Liu,Emilia Entcheva

Physiological reports 11:e15696 PubMed37226398

2023

Inhibition of Wnt/β-catenin signaling upregulates Na 1.5 channels in Brugada syndrome iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Aizhu Lu,Ruonan Gu,Cencen Chu,Ying Xia,Jerry Wang,Darryl R Davis,Wenbin Liang

iScience 25:104573 PubMed35789851

2022

knockout mice show autistic-like behaviors and aberrant synapse formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuda Huo,Wen Lu,Yuan Tian,Qingming Hou,Heng-Ye Man

Methods in molecular biology (Clifton, N.J.) 2485:15-37 PubMed35618896

2022

Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates.

Applications

Unspecified application

Species

Unspecified reactive species

Weizhen Li,Julie L Han,Emilia Entcheva

Nature medicine 27:954-963 PubMed34083813

2021

Biomarkers for neurodegenerative diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Oskar Hansson

American journal of physiology. Heart and circulatory physiology 319:H1112-H1122 PubMed32986966

2020

Syncytium cell growth increases Kir2.1 contribution in human iPSC-cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Weizhen Li,Julie L Han,Emilia Entcheva

EBioMedicine 54:102723 PubMed32259713

2020

Identification, clinical manifestation and structural mechanisms of mutations in AMPK associated cardiac glycogen storage disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Hu,Dong Hu,Liwen Liu,Daniel Barr,Yang Liu,Norma Balderrabano-Saucedo,Bo Wang,Feng Zhu,Yumei Xue,Shulin Wu,BaoLiang Song,Heather McManus,Katherine Murphy,Katherine Loes,Arnon Adler,Lorenzo Monserrat,Charles Antzelevitch,Michael H Gollob,Perry M Elliott,Hector Barajas-Martinez

Biomedicines 8: PubMed32183098

2020

Unraveling the Role of Inwardly Rectifying Potassium Channels in the Hippocampus of an Aβ-Infused Rat Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Enes Akyuz,Chiara Villa,Merve Beker,Birsen Elibol

British journal of pharmacology 176:2780-2794 PubMed31074016

2019

Contribution of potassium channels to action potential repolarization of human embryonic stem cell-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Wang,Renjun Zhu,Leslie Tung

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Kir2.1/KCNJ2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody (AB65796)

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody (AB65796)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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