AB109750

Anti-Kir2.1/KCNJ2 antibody [EPR4530]

Name: Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750) | Abcam
Brand: Abcam Limited
SKU: AB109750
Price: 220 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(12 Publications)

Rabbit Recombinant Monoclonal Kir2.1/KCNJ2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 12 publications.

View Alternative Names

IRK1, KCNJ2, Inward rectifier potassium channel 2, Cardiac inward rectifier potassium channel, Inward rectifier K(+) channel Kir2.1, IRK-1, hIRK1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

ab109750 at 1/100 dilution staining Kir2.1/KCNJ2 in SH SY5Y cells by Immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

ab109750 at 1/250 dilution staining Kir2.1/KCNJ2 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Lab

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

In Western blot, Anti-Integrin alpha V antibody [EPR16800] was used as membrane Control (ab179475) at 1 : 5,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 membrane lysate at 20 µg

Lane 3:

HEK-293 cytoplasm lysate at 20 µg

Lane 4:

HEK-293 nuclear lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 20s

Lab

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative sample : human lung (PMID : 25880778).

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750) at 1/1000 dilution

Lane 1:

human lung (PMID: 25880778) at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 4:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 20s

Unknown

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Predicted band size: 48 kDa

false

Carrier free

Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4530

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Kir2.1 protein also known as KCNJ2 belongs to a family of inward-rectifier potassium channels. Kir2.1 is important for setting and stabilizing the resting membrane potential. It has a molecular mass of approximately 48 kDa. Kir2.1 is mainly expressed in the heart and brain tissues and it facilitates the flow of potassium ions across the cell membrane with high efficiency. This modulation of potassium ion movement is critical for maintaining membrane potential and is vital for the normal electrical activity in excitable tissues.

Biological function summary

This process aids in the regulation of cellular excitability. Kir2.1 is an integral part of cell membranes and often associates with other proteins to form a functional channel complex. This complex plays a role in neuronal signaling and cardiac rhythm regulation contributing to various physiological functions in muscle and nerve tissue. Its roles underpin how potassium currents modulate nerve impulses and heartbeats reflecting its participation in the body's complex communication and control systems.

Pathways

The Kir2.1 channel influences the cardiac action potential and is involved in maintaining the stability of the heart’s electrical signaling. It is part of the cardiac conduction pathway. The channel interacts with other cardiac proteins like KCNJ3 and KCNJ5 complementing their function in the repolarization phase of the cardiac action potential. Kir2.1's presence in pathways responsible for repolarization highlights the precision needed for effective cardiac and neurological functions.

Mutations in the Kir2.1 gene KCNJ2 relate to Andersen-Tawil syndrome and cardiac arrhythmias. Andersen-Tawil syndrome characterized by periodic paralysis cardiac arrhythmias and developmental anomalies highlights the multifaceted role Kir2.1 plays in cellular function. Kir2.1 mutations disrupt electrical stability in heart and skeletal muscle tissues. Similar mutations associate with connections to other membrane proteins like KCNH2 often exacerbating arrhythmic risk a critical factor in heart-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it (PubMed : 36149965, PubMed : 7590287, PubMed : 9490857). Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages (PubMed : 7590287, PubMed : 7696590). The inward rectification is mainly due to the blockage of outward current by internal magnesium (PubMed : 9490857). Can be blocked by extracellular barium or cesium (PubMed : 7590287, PubMed : 7696590). Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues (PubMed : 7590287, PubMed : 7696590, PubMed : 7840300).

See full target information KCNJ2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 326:C1543-C1555 PubMed38586877

2024

Visceral adipose of obese mice inhibits endothelial inwardly rectifying K channels in a CD36-dependent fashion.

Applications

Unspecified application

Species

Unspecified reactive species

Sabita Rokka,Masoumeh Sadeghinejad,Emma C Hudgins,Erica J Johnson,Thanh Nguyen,Ibra S Fancher

Frontiers in physiology 14:1124444 PubMed36814472

2023

Na and K transport and maturation stage ameloblast modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jake Ngu,Antonius L J J Bronckers,Kaitlin Katsura,Yan Zhang,Pamela K Den Besten

Nature cardiovascular research 1:900-917 PubMed39195979

2022

Kir2.1 dysfunction at the sarcolemma and the sarcoplasmic reticulum causes arrhythmias in a mouse model of Andersen-Tawil syndrome type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Álvaro Macías,Andrés González-Guerra,Ana I Moreno-Manuel,Francisco M Cruz,Lilian K Gutiérrez,Nieves García-Quintáns,Marta Roche-Molina,Francisco Bermúdez-Jiménez,Vicente Andrés,María Linarejos Vera-Pedrosa,Isabel Martínez-Carrascoso,Juan A Bernal,José Jalife

International journal of molecular medicine 50: PubMed35856410

2022

Inhibition of KIR2.1 decreases pulmonary artery smooth muscle cell proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Cao,Nigala Aikeremu,Wen-Yan Shi,Xue-Chun Tang,Rui-Juan Gao,Liang-Jing-Yuan Kong,Jing-Rong Zhang,Wen-Juan Qin,Ai-Mei Zhang,Ke-Tao Ma,Li Li,Jun-Qiang Si

Experimental physiology 107:589-600 PubMed35363405

2022

α -Adrenoceptors activate the NLRP3 inflammasome through downregulation of Kir2.1 in cardiac inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Wenbo Wang,Xiangyang Peng,Luqian Liu,Aimei Zhang,Xinzhi Li,Ketao Ma,Li Wang

Signal transduction and targeted therapy 7:72 PubMed35273141

2022

Elevated Kir2.1/nuclear N2ICD defines a highly malignant subtype of non-WNT/SHH medulloblastomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Xia Wang,Haibo Wu,Yong Ren,Shengqing Lv,Chengdong Ji,Dongfang Xiang,Mengsi Zhang,Huimin Lu,Wenjuan Fu,Qing Liu,Zexuan Yan,Qinghua Ma,Jingya Miao,Ruili Cai,Xi Lan,Bin Wu,Wenying Wang,Yinhua Liu,Dai-Zhong Wang,Mianfu Cao,Zhicheng He,Yu Shi,Yifang Ping,Xiaohong Yao,Xia Zhang,Peng Zhang,Ji Ming Wang,Yan Wang,Youhong Cui,Xiu-Wu Bian

Molecular medicine reports 24: PubMed34212982

2021

Interference with KCNJ2 inhibits proliferation, migration and EMT progression of apillary thyroid carcinoma cells by upregulating GNG2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Chen,Miaoming Huang,Xiarong Hu

Cell stem cell 24:802-811.e5 PubMed30880024

2019

A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles.

Applications

Unspecified application

Species

Unspecified reactive species

Joe Z Zhang,Vittavat Termglinchan,Ning-Yi Shao,Ilanit Itzhaki,Chun Liu,Ning Ma,Lei Tian,Vicky Y Wang,Alex C Y Chang,Hongchao Guo,Tomoya Kitani,Haodi Wu,Chi Keung Lam,Kazuki Kodo,Nazish Sayed,Helen M Blau,Joseph C Wu

JCI insight 3: PubMed30282820

2018

Reverse electrical remodeling in rats with heart failure and preserved ejection fraction.

Applications

Species

Rat

Jae Hyung Cho,Peter J Kilfoil,Rui Zhang,Ryan E Solymani,Catherine Bresee,Elliot M Kang,Kristin Luther,Russell G Rogers,Geoffrey de Couto,Joshua I Goldhaber,Eduardo Marbán,Eugenio Cingolani

Journal of the American Heart Association 7: PubMed29502106

2018

Hypercholesterolemia-Induced Loss of Flow-Induced Vasodilation and Lesion Formation in Apolipoprotein E-Deficient Mice Critically Depend on Inwardly Rectifying K Channels.

Applications

Species

Mouse

Ibra S Fancher,Sang Joon Ahn,Crystal Adamos,Catherine Osborn,Myung-Jin Oh,Yun Fang,Catherine A Reardon,Godfrey S Getz,Shane A Phillips,Irena Levitan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kir2.1/KCNJ2 antibody [EPR4530]

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (AB109750)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise