AB239988

Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Kir2.1/KCNJ2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IRK1, KCNJ2, Inward rectifier potassium channel 2, Cardiac inward rectifier potassium channel, Inward rectifier K(+) channel Kir2.1, IRK-1, hIRK1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

ab109750 at 1/250 dilution staining Kir2.1/KCNJ2 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109750).

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

ab109750 at 1/100 dilution staining Kir2.1/KCNJ2 in SH SY5Y cells by Immunofluorescence.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109750).

Lab

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

This data was developed using ab109750, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Integrin alpha V antibody [EPR16800] was used as membrane Control (ab179475) at 1 : 5,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (<a href='/en-us/products/primary-antibodies/kir21-kcnj2-antibody-epr4530-ab109750'>ab109750</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 membrane lysate at 20 µg

Lane 3:

HEK-293 cytoplasm lysate at 20 µg

Lane 4:

HEK-293 nuclear lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 20s

Lab

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

This data was developed using ab109750, the same antibody clone in a different buffer formulation.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative sample : human lung (PMID : 25880778).

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] (<a href='/en-us/products/primary-antibodies/kir21-kcnj2-antibody-epr4530-ab109750'>ab109750</a>) at 1/1000 dilution

Lane 1:

human lung (PMID: 25880778) at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 4:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 20s

Unconjugated

Anti-Kir2.1/KCNJ2 antibody [EPR4530]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4530

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab239988 is the carrier-free version of ab109750.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Kir2.1 protein also known as KCNJ2 belongs to a family of inward-rectifier potassium channels. Kir2.1 is important for setting and stabilizing the resting membrane potential. It has a molecular mass of approximately 48 kDa. Kir2.1 is mainly expressed in the heart and brain tissues and it facilitates the flow of potassium ions across the cell membrane with high efficiency. This modulation of potassium ion movement is critical for maintaining membrane potential and is vital for the normal electrical activity in excitable tissues.

Biological function summary

This process aids in the regulation of cellular excitability. Kir2.1 is an integral part of cell membranes and often associates with other proteins to form a functional channel complex. This complex plays a role in neuronal signaling and cardiac rhythm regulation contributing to various physiological functions in muscle and nerve tissue. Its roles underpin how potassium currents modulate nerve impulses and heartbeats reflecting its participation in the body's complex communication and control systems.

Pathways

The Kir2.1 channel influences the cardiac action potential and is involved in maintaining the stability of the heart’s electrical signaling. It is part of the cardiac conduction pathway. The channel interacts with other cardiac proteins like KCNJ3 and KCNJ5 complementing their function in the repolarization phase of the cardiac action potential. Kir2.1's presence in pathways responsible for repolarization highlights the precision needed for effective cardiac and neurological functions.

Mutations in the Kir2.1 gene KCNJ2 relate to Andersen-Tawil syndrome and cardiac arrhythmias. Andersen-Tawil syndrome characterized by periodic paralysis cardiac arrhythmias and developmental anomalies highlights the multifaceted role Kir2.1 plays in cellular function. Kir2.1 mutations disrupt electrical stability in heart and skeletal muscle tissues. Similar mutations associate with connections to other membrane proteins like KCNH2 often exacerbating arrhythmic risk a critical factor in heart-related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it (PubMed : 36149965, PubMed : 7590287, PubMed : 9490857). Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages (PubMed : 7590287, PubMed : 7696590). The inward rectification is mainly due to the blockage of outward current by internal magnesium (PubMed : 9490857). Can be blocked by extracellular barium or cesium (PubMed : 7590287, PubMed : 7696590). Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues (PubMed : 7590287, PubMed : 7696590, PubMed : 7840300).

See full target information KCNJ2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:554831 PubMed34336814

2021

Transcription Factor DLX5 Promotes Hair Follicle Stem Cell Differentiation by Regulating the c-MYC/microRNA-29c-3p/NSD1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bojie Lin,Jiangying Zhu,Guoqian Yin,Mingde Liao,Guanyu Lin,Yuyong Yan,Dan Huang,Siding Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

Western blot - Anti-Kir2.1/KCNJ2 antibody [EPR4530] - BSA and Azide free (AB239988)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise