Anti-Kir2.1/KCNJ2 antibody [S112]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (AB85492)

ab85492 staining Kir2.1/KCNJ2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

Incubated with primary antibody at 1 : 1000 for 1 hour at RT. Secondary Antibody : FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [S112] (AB85492)

ICC/IF image of ab85492 stained SK-N-SH (Human neuroblastoma cell line) cells.

The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85492, 10 μg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight^® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

• Flow Cyt

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Flow Cytometry - Anti-Kir2.1/KCNJ2 antibody [S112] (AB85492)

Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab85492 (red line).

The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85492, 1 μg/1x10⁶ cells) for 30 minutes at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween for 20 minutes used under the same conditions.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [S112] (AB85492)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Kir2.1/KCNJ2 with ab85492 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (AB85492)

ab85492 staining Kir2.1/KCNJ2 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded).

Incubated with primary antibody at 1 : 1000 for 1 hour at RT. Secondary Antibody : FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT.

• WB

Supplier Data

Western blot - Anti-Kir2.1/KCNJ2 antibody [S112] (AB85492)

All lanes:

Western blot - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492) at 1/1000 dilution

All lanes:

Monkey COS transient cell lysate at 15 µg

Secondary

All lanes:

Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Predicted band size: 48 kDa

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