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AB252936

Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92]

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(1 Publication)

Rabbit Recombinant Monoclonal KIR2DL1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

View Alternative Names

CD158a, CD158A, NKAT1, KIR2DL1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, p58 natural killer cell receptor clones CL-42/47.11, p58.1 MHC class-I-specific NK receptor, NKAT-1, p58 NK receptor CL-42/47.11

3 Images
Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (AB252936)
  • Flow Cyt

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Flow Cytometry - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (AB252936)

Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling KIR2DL1 + KIR2DL2 with ab252936 at 1/50 dilution (Right) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left).

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab252936 (Right). Then stained with anti-CD56 conjugated to BV421.

Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (AB252936)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (AB252936)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 + KIR2DL2 with a252936 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in natural killer (NK) cells in human endometrium (PMID : 7749980) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Western blot - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (AB252936)
  • WB

Unknown

Western blot - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (AB252936)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KIR2DL1 + KIR2DL2 antibody [EPR22492-92] (ab252936) at 1/1000 dilution

Lane 1:

His-tagged human KIR2DL1 recombinant protein (aa1-245) 20 ng

Lane 2:

His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221) 20 ng

Lane 3:

His-tagged human KIR2DL3 recombinant protein (aa1-245) 20 ng

Lane 4:

His-tagged human KIR2DS1 recombinant protein (aa22-221) 20 ng

Lane 5:

His-tagged human KIR2DS2 recombinant protein (aa22-221) 20 ng

Lane 6:

His-tagged human KIR2DS3 recombinant protein (aa22-221) 20 ng

Lane 7:

His-tagged human KIR2DS4 recombinant protein (aa22-221) 20 ng

Lane 8:

His-tagged human KIR2DS5 recombinant protein (aa22-221) 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22492-92

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor on natural killer (NK) cells for some HLA-C alleles such as w4 and w6. Inhibits the activity of NK cells thus preventing cell lysis.
See full target information KIR2DL1

Additional targets

KIR2DL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:15988-16000 PubMed40730713

2025

Effects and Mechanisms of NMDAR-EphB2 on Synapses in Mice by Active Immunization with a GluN1 Peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Qian,Youshi Meng,Lin Zhang,Lanfeng Sun,Sijie Ou,Rida Li,Chi Gong,Shengyu Yang,Yuan Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com