Rabbit Recombinant Monoclonal KIR2DL1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.
Images
![Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--western-blot-img179627.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697)")
![Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--western-blot-img28960.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697)")
![Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--flow-cytometry-img131219.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131947.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (AB224697)")
Publications
Filter by
- Scientific reports 12:67692022Blockade of inhibitory killer cell immunoglobulin-like receptors and IL-2 triggering reverses the functional hypoactivity of tumor-derived NK-cells in glioblastomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCüneyt Sönmez,Johannes Wölfer,Markus Holling,Benjamin Brokinkel,Walter Stummer,Heinz Wiendl,Christian Thomas,Andreas Schulte-Mecklenbeck,Oliver M GrauerPubMed 35474089
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Receptor on natural killer (NK) cells for some HLA-C alleles such as w4 and w6. Inhibits the activity of NK cells thus preventing cell lysis.
Additional Targets
KIR2DL1, KIR2DL3
Alternative names
CD158a, CD158A, NKAT1, KIR2DL1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, p58 natural killer cell receptor clones CL-42/47.11, p58.1 MHC class-I-specific NK receptor, NKAT-1, p58 NK receptor CL-42/47.11
Recommended products
Rabbit Recombinant Monoclonal KIR2DL1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22541-47
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--western-blot-img179627.jpg "Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)")
Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)
Blocking and diluting buffer: 5% NFDM/TBST
The actual band size higher than predicted is mainly due to glycosylation.
The molecular weight observed is consistent with what has been described in the literatures
(PMID:11454312 and PMID:12370356).
All lanes: Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697) at 1/2000 dilution
All lanes: HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 38-60 kDa
Exposure time: 40s
![Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--western-blot-img28960.jpg "Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)")
Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697) at 1/1000 dilution
Lane 1: His-tagged human KIR2DL1 recombinant protein (aa1-245)
Lane 2: His and LIF-tagged human KIR2DL2 recombinant protein (aa26-221)
Lane 3: His-tagged human KIR2DL3 recombinant protein (aa1-245)
Lane 4: His-tagged human KIR2DS1 recombinant protein (aa22-221)
Lane 5: His-tagged human KIR2DS2 recombinant protein (aa22-221)
Lane 6: His-tagged human KIR2DS3 recombinant protein (aa22-221)
Lane 7: His-tagged human KIR2DS4 recombinant protein (aa22-221)
Lane 8: His-tagged human KIR2DS5 recombinant protein (aa22-221) 20 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 15s
![Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--flow-cytometry-img131219.jpg "Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)")
Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/600 compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab224697 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697), expandable thumbnail](https://content.abcam.com/products/images/kir2dl3-kir2dl1-kir2dl2-antibody-epr22541-47-ab224697--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131947.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DL2 antibody [EPR22541-47] (ab224697)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL2+KIR2DL1+KIR2DL3 with ab224697 at 1/500 dilution (1.13μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID: 7749980). Counterstained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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