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AB255327

Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal KIR2DL1 antibody. Carrier free. Suitable for WB, Flow Cyt, IHC-P and reacts with Recombinant fragment - Human, Human samples. Cited in 1 publication.

View Alternative Names

CD158a, CD158A, NKAT1, KIR2DL1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, p58 natural killer cell receptor clones CL-42/47.11, p58.1 MHC class-I-specific NK receptor, NKAT-1, p58 NK receptor CL-42/47.11, CD158b2, CD158B2, KIRCL23, NKAT2, KIR2DL3, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, p58 natural killer cell receptor clone CL-6, p58.2 MHC class-I-specific NK receptor, NKAT-2, p58 NK receptor CL-6, CD158b1, CD158B1, NKAT6, KIR2DL2, Killer cell immunoglobulin-like receptor 2DL2, CD158 antigen-like family member B1, Natural killer-associated transcript 6, p58 natural killer cell receptor clone CL-43, NKAT-6, p58 NK receptor CL-43, CD158j, CD158J, NKAT5, KIR2DS2, Killer cell immunoglobulin-like receptor 2DS2, CD158 antigen-like family member J, NK receptor 183 ActI, Natural killer-associated transcript 5, p58 natural killer cell receptor clone CL-49, NKAT-5, p58 NK receptor CL-49, CD158h, CD158H, KIR2DS1, Killer cell immunoglobulin-like receptor 2DS1, CD158 antigen-like family member H, MHC class I NK cell receptor Eb6 ActI

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human colon cancer (PMID : 7749980) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human gastric cancer (PMID : 7749980) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR2DL1 with ab252938 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in human endometrium (PMID : 7749980) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64] - BSA and Azide free (AB255327)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling KIR2DL1 with ab252938 at 1/50 (right) compared with a Rabbit monoclonal IgG (ab172730) (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097), at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab252938 (Right). Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252938).

  • Unconjugated

    Anti-KIR2DL3 + KIR2DL1 + KIR2DS2 + KIR2DL2 + KIR2DS1 antibody [EPR22492-64]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22492-64

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval using <a href='/en-us/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a> (Tris/EDTA buffer, pH 9.0).</p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab255327 is the carrier-free version of ab252938.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor on natural killer (NK) cells for some HLA-C alleles such as w4 and w6. Inhibits the activity of NK cells thus preventing cell lysis.
See full target information KIR2DL1

Additional targets

KIR2DL2,KIR2DL3,,
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of the rheumatic diseases 83:926-944 PubMed38325908

2024

Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Fan,Xuzhao Bian,Xiaogao Meng,Lei Li,Laiyi Fu,Yanan Zhang,Long Wang,Yan Zhang,Dalong Gao,Xiong Guo,Mikko Juhani Lammi,Guangdun Peng,Shiquan Sun
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com