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Rabbit Recombinant Monoclonal KIR3DL1 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Transfected cell line - Human, Human, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (AB259808), expandable thumbnail
  • Flow Cytometry - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (AB259808), expandable thumbnail
  • Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (AB259808), expandable thumbnail
  • Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (AB259808), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (AB259808), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Expected
Expected
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell line - Human
Tested
Tested
Not recommended
Tested
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Mouse, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Target data

Function

Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.

Additional Targets

KIR3DS1, KIR2DS2, KIR2DS4

Alternative names

Recommended products

Rabbit Recombinant Monoclonal KIR3DL1 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Transfected cell line - Human, Human, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24357-139
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808), expandable thumbnail

    Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern & observed MW are consistent with what has been described in the literature (PMID:17911614).

    Negative control: human tonsil (PMID:14734722)

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808) at 1/1000 dilution

    Lane 1: Human spleen tissue lysate at 60 µg

    Lane 2: Human tonsil tissue lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 60-80 kDa

  • Flow Cytometry - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808), expandable thumbnail

    Flow Cytometry - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling KIR3DL1+KIR3DS1+KIR2DS2+KIR2DS4 with ab259808 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab259808. Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.

  • Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808), expandable thumbnail

    Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody has high cross-reactivity with human KIR2DS4, and low with human KIR2DS2. This antibody does not recognize human KIR3DL3.

    All of the rec proteins were made in-house. KIR3DL1 and KIR2DS2 were expressed from HEK-293 expression systems. KIR3DL3 and KIR2DS4 were expressed from E.coli expression systems.

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808) at 1/1000 dilution

    Lane 1: His-tagged human KIR3DL1 recombinant protein at 0.01 µg

    Lane 2: His-tagged human KIR3DL3 recombinant protein at 0.01 µg

    Lane 3: His-tagged human KIR2DS4 recombinant protein at 0.01 µg

    Lane 4: LIF/His-tagged human KIR2DS2 recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 33-87 kDa

  • Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808), expandable thumbnail

    Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody can recognize human KIR3DS1 but can not recognize human KIR3DL2.

    Exposure time: Lane 1-4: 3 minutes Lane 5-6: 59 seconds

    All lanes: Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808) at 1/1000 dilution

    Lanes 1, 3 and 5: 293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: 293T transfected with human KIR3DL2 expression vector containing a myc-His-tag®, whole cell lysate 20 at 20 µg

    Lane 6: 293T transfected with human KIR3DS1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45-70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney) cells labelling KIR3DL1+KIR3DS1+KIR2DS2+KIR2DS4 with ab259808 at 1/50 dilution (10.2 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged KIR3DL1 expression vector is observed.

    Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain myc-tag at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

  • Immunoprecipitation - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808), expandable thumbnail

    Immunoprecipitation - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    KIR3DL1+KIR3DS1+KIR2DS2+KIR2DS4 was immunoprecipitated from 0.35 mg 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate with ab259808 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259808 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: 293T (human embryonic kidney) transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate 10 ug

    Lane 2: ab259808 IP in 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259808 in 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    All lanes: Immunoprecipitation - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)

    Observed band size: 60-80 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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