Rabbit Recombinant Monoclonal KIR3DL1 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Transfected cell line - Human, Human, Recombinant full length protein - Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ICC/IF | IP | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|---|
Human | Expected | Expected | Tested | Tested | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Transfected cell line - Human | Tested | Tested | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Transfected cell line - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.
KIR3DS1, KIR2DS2, KIR2DS4
CD158e, CD158E, NKAT3, NKB1, KIR3DL1, Killer cell immunoglobulin-like receptor 3DL1, CD158 antigen-like family member E, HLA-BW4-specific inhibitory NK cell receptor, Natural killer-associated transcript 3, p70 natural killer cell receptor clones CL-2/CL-11, NKAT-3, p70 NK receptor CL-2/CL-11
Rabbit Recombinant Monoclonal KIR3DL1 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Transfected cell line - Human, Human, Recombinant full length protein - Human samples.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression pattern & observed MW are consistent with what has been described in the literature (PMID:17911614).
Negative control: human tonsil (PMID:14734722)
Exposure time: 3 minutes
All lanes: Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 60 µg
Lane 2: Human tonsil tissue lysate at 60 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 60-80 kDa
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling KIR3DL1+KIR3DS1+KIR2DS2+KIR2DS4 with ab259808 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab259808. Then stained with anti-CD56 conjugated to BV421. Gated on viable cells.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody has high cross-reactivity with human KIR2DS4, and low with human KIR2DS2. This antibody does not recognize human KIR3DL3.
All of the rec proteins were made in-house. KIR3DL1 and KIR2DS2 were expressed from HEK-293 expression systems. KIR3DL3 and KIR2DS4 were expressed from E.coli expression systems.
Exposure time: 15 seconds
All lanes: Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808) at 1/1000 dilution
Lane 1: His-tagged human KIR3DL1 recombinant protein at 0.01 µg
Lane 2: His-tagged human KIR3DL3 recombinant protein at 0.01 µg
Lane 3: His-tagged human KIR2DS4 recombinant protein at 0.01 µg
Lane 4: LIF/His-tagged human KIR2DS2 recombinant protein at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33-87 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody can recognize human KIR3DS1 but can not recognize human KIR3DL2.
Exposure time: Lane 1-4: 3 minutes Lane 5-6: 59 seconds
All lanes: Western blot - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808) at 1/1000 dilution
Lanes 1, 3 and 5: 293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: 293T transfected with human KIR3DL2 expression vector containing a myc-His-tag®, whole cell lysate 20 at 20 µg
Lane 6: 293T transfected with human KIR3DS1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45-70 kDa
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney) cells labelling KIR3DL1+KIR3DS1+KIR2DS2+KIR2DS4 with ab259808 at 1/50 dilution (10.2 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged KIR3DL1 expression vector is observed.
Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain myc-tag at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.
KIR3DL1+KIR3DS1+KIR2DS2+KIR2DS4 was immunoprecipitated from 0.35 mg 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate with ab259808 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259808 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney) transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate 10 ug
Lane 2: ab259808 IP in 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259808 in 293T transfected with human KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
All lanes: Immunoprecipitation - Anti-KIR3DL1 + KIR2DS4 + KIR2DS2 + KIR3DS1 antibody [EPR24357-139] (ab259808)
Observed band size: 60-80 kDa
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