Rabbit Recombinant Monoclonal KIR3DL1 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423405.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423403.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423402.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423401.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (AB314869)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | WB | IP | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.
Alternative names
Killer cell immunoglobulin-like receptor 3DL1, CD158 antigen-like family member E, HLA-BW4-specific inhibitory NK cell receptor, MHC class I NK cell receptor, Natural killer-associated transcript 3, p70 natural killer cell receptor clones CL-2/CL-11, NKAT-3, p70 NK receptor CL-2/CL-11, KIR3DL1, CD158E, NKAT3, NKB1
Recommended products
Rabbit Recombinant Monoclonal KIR3DL1 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples.
Alternative names
Killer cell immunoglobulin-like receptor 3DL1, CD158 antigen-like family member E, HLA-BW4-specific inhibitory NK cell receptor, MHC class I NK cell receptor, Natural killer-associated transcript 3, p70 natural killer cell receptor clones CL-2/CL-11, NKAT-3, p70 NK receptor CL-2/CL-11, KIR3DL1, CD158E, NKAT3, NKB1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR27252-40
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab314869 is the carrier-free version of Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423405.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)
This data was developed using Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human KIR3DL1 expression vector containing a his tag. (B) HEK-293T transfected with a human KIR2DL3 expression vector containing a his tag. (C) HEK-293T transfected with a human KIR3DL2 expression vector containing a his tag. (D) HEK-293T transfected with a human KIR3DS1 expression vector containing a his tag. (E) HEK-293T transfected with empty vector containing a His tag. tissue labeling KIR3DL1+KIR2DL3+KIR3DL2 with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 at 1/4000 (0.129 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a human KIR3DL1 expression vector containing a his tag, (B) HEK-293T transfected with a human KIR2DL3 expression vector containing a his tag, and (C) HEK-293T transfected with a human KIR3DL2 expression vector containing a his tag. No staining on (D) HEK-293T transfected with a human KIR3DS1 expression vector containing a his tag and (E) HEK-293T transfected with empty vector containing a His tag. The section was incubated with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)
This data was developed using Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling KIR3DL1+KIR2DL3+KIR3DL2 with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human kidney. The section was incubated with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423403.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)
This data was developed using Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling KIR3DL1+KIR2DL3+KIR3DL2 with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human cerebrum. The section was incubated with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423402.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)
This data was developed using Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human NK/T cell lymphoma tissue labeling KIR3DL1+KIR2DL3+KIR3DL2 with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human NK/T cell lymphoma. The section was incubated with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869), expandable thumbnail](https://content.abcam.com/products/images/kir3dl1kir2dl3kir3dl2-antibody-epr27252-40-bsa-and-azide-free-ab314869--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423401.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] - BSA and Azide free (ab314869)
This data was developed using Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR2DL3+KIR3DL2 with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 at 1/100 (5.14 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on immune cells of human endometrium. The section was incubated with Anti-KIR3DL1+KIR2DL3+KIR3DL2 antibody [EPR27252-40] ab314868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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