AB240876

Anti-Kir4.1/KCNJ10 antibody

Name: Anti-Kir4.1/KCNJ10 antibody (ab240876) | Abcam
Brand: Abcam Limited
SKU: AB240876
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Kir4.1/KCNJ10 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human KCNJ10.

View Alternative Names

ATP-sensitive inward rectifier potassium channel 10, ATP-dependent inwardly rectifying potassium channel Kir4.1, Inward rectifier K(+) channel Kir1.2, KCNJ10

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (AB240876)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (AB240876)

Immunocytochemistry/Immunofluorescence analysis of RKO (human colon carcinoma cell line) cells labeling Kir4.1/KCNJ10 using ab240876 (green) at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.

(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240876 (D) Composite.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (AB240876)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (AB240876)

Immunohistochemistry of human kidney tissue staining Kir4.1/KCNJ10 using ab240876 at (1/50) for 30 min at room temperature.

Supplier Data

Western blot - Anti-Kir4.1/KCNJ10 antibody (AB240876)

Blocking Buffer : 5% Skim milk in TBST, incubated 1h at RT. 12% SDS-PAGE.

Lane 1:

Western blot - Anti-Kir4.1/KCNJ10 antibody (ab240876) at 1 µg/mL

Lane 2:

None, TBST only

All lanes:

Rat liver, no heat at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG:HRP at 1/4000 dilution

Predicted band size: 42 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human KCNJ10. The exact immunogen used to generate this antibody is proprietary information.

P78508

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kir4.1 also known as KCNJ10 is an inwardly rectifying potassium channel. It is a protein with a mass of approximately 45 kDa. Kir4.1 facilitates the movement of potassium ions across the cell membrane helping maintain the membrane potential in certain cells. This channel prominently expresses in the brain kidney and inner ear. In glial cells of the central nervous system it plays an important role in maintaining potassium homeostasis. This is important for the correct functioning of neurons and other glial cells.

Biological function summary

The potassium channel Kir4.1 functions to balance potassium levels in the extracellular space which is important for nerve impulse propagation and muscle contraction. Kir4.1 frequently forms a complex with another potassium channel Kir5.1 (KCNJ16). Together these complexes contribute to fine-tuning the electrochemical gradient impacting various cell types. Its distribution and activity affect neuronal signaling renal function and endolymph generation in the ear.

Pathways

Kir4.1 plays essential roles in neural signaling pathways and renal reabsorption processes. This target interacts with proteins like AQP4 in the brain influencing water transport and ion homeostasis. Its functionality is part of the regulatory mechanisms within the distal convoluted tubule of the kidney impacting water and salt balance. These interactions facilitate proper electrical signaling and osmoregulation in the body.

Kir4.1 has associations with disorders such as epilepsy and EAST syndrome. Defects or dysregulation in the Kir4.1 channel can disrupt potassium ion balance which may lead to abnormal neuronal excitability manifesting as epilepsy. Additionally mutations in this channel can contribute to EAST syndrome a condition involving epilepsy ataxia sensorineural deafness and renal tubulopathy. Related proteins like AQP4 can also be implicated highlighting the complexity of physiological processes involving Kir4.1.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be responsible for potassium buffering action of glial cells in the brain (By similarity). Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it (PubMed : 8995301). Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages (PubMed : 8995301). The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (PubMed : 8995301). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (PubMed : 24561201).

See full target information KCNJ10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 16:896172 PubMed36060277

2022

Astrocytes profiling in acute hepatic encephalopathy: Possible enrolling of glial fibrillary acidic protein, tumor necrosis factor-alpha, inwardly rectifying potassium channel (Kir 4.1) and aquaporin-4 in rat cerebral cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Dalia Mahmoud Abdelmonem Elsherbini,Fatma M Ghoneim,Eman Mohammed El-Mancy,Hasnaa Ali Ebrahim,Mohamed El-Sherbiny,Mohamed El-Shafey,Rasha Hamed Al-Serwi,Nehal M Elsherbiny

Molecular neurobiology 58:1550-1563 PubMed33215390

2020

Ginsenoside Rg1 Prevents PTSD-Like Behaviors in Mice Through Promoting Synaptic Proteins, Reducing Kir4.1 and TNF-α in the Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengrong Zhang,Zhujin Song,Fengming Shen,Pan Xie,Juan Wang,Ai-Song Zhu,Guoqi Zhu

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Kir4.1/KCNJ10 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Kir4.1/KCNJ10 antibody (AB240876)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir4.1/KCNJ10 antibody (AB240876)

Western blot - Anti-Kir4.1/KCNJ10 antibody (AB240876)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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