AB306551

Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Kir4.1/KCNJ10 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.

View Alternative Names

ATP-sensitive inward rectifier potassium channel 10, Inward rectifier K(+) channel Kir4.1, Kcnj10

9 Images

Supplier Data

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Kir4.1/KCNJ10 with ab306550 at 1/500 (1.0 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on rat cardiac muscle. The section was incubated with ab306550 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Kir4.1/KCNJ10 with ab306550 at 1/500 (1.0 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : No staining on mouse cardiac muscle. The section was incubated with ab306550 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Kir4.1/KCNJ10 with ab306550 at 1/500 (1.0 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on distal tubules in rat kidney (PMID : 8654579). The section was incubated with ab306550 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Kir4.1/KCNJ10 with ab306550 at 1/500 (1.0 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on stellate cells in mouse cerebrum (PMID : 11169792). The section was incubated with ab306550 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Supplier Data

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1 (PMID : 33348803; PMID : 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10. Samples are non-boiled as boiling may cause protein aggregation. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] (<a href='/en-us/products/primary-antibodies/kir41-kcnj10-antibody-epr27251-79-ab306550'>ab306550</a>) at 1/1000 dilution

Lane 1:

Mouse brain non-membrane fraction 20 μg

Lane 2:

Mouse brain membrane fraction 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 250 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Samples are non-boiled as boiling may cause protein aggregation. Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1 (PMID : 33348803; PMID : 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10. 70 seconds Exposure time :

All lanes:

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] (<a href='/en-us/products/primary-antibodies/kir41-kcnj10-antibody-epr27251-79-ab306550'>ab306550</a>) at 1/1000 dilution

Lane 1:

Rat brain non-membrane fraction 40 μg

Lane 2:

Rat brain membrane fraction 40 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. Samples are non-boiled as boiling may cause protein aggregation. Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1 (PMID : 33348803; PMID : 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10. Exposure time : 70 seconds

All lanes:

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] (<a href='/en-us/products/primary-antibodies/kir41-kcnj10-antibody-epr27251-79-ab306550'>ab306550</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate 40 μg

Lane 2:

Rat heart tissue lysate 40 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Kir4.1/KCNJ10 is specifically expressed in the brain and kidney (PMID : 11169792; PMID : 15310750; PMID : 11169792; PMID : 24561201).

Samples are non-boiled as boiling may cause protein aggregation.

Kir4.1/KCNJ10 is expressed in vivo as a homotetramer (four units of KIR4.1) or a heterotetramer with KIR5.1(two units of KIR4.1 and KIR5.1). (PMID : 33348803; PMID : 15310750 ). This antibody can only detect the tetramer of Kir4.1/KCNJ10.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Exposure time :

Lanes 1, 4 and 5 : 180 seconds

Lanes 2 and 3 : 37 seconds

All lanes:

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] (<a href='/en-us/products/primary-antibodies/kir41-kcnj10-antibody-epr27251-79-ab306550'>ab306550</a>) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse kidney lysate at 20 µg

Lane 5:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 250 kDa

true

Supplier Data

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

This data was developed using ab306550, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody does not cross-react with human Kir4.2/KCNJ15 and human Kir1.1/KCNJ1. Exposure time : 15 seconds.

All lanes:

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] (<a href='/en-us/products/primary-antibodies/kir41-kcnj10-antibody-epr27251-79-ab306550'>ab306550</a>) at 1/1000 dilution

Lane 1:

His-tagged human Kir4.1/KCNJ10 fragment 10 ng

Lane 2:

GST-tagged human Kir4.2/KCNJ15 recombinant protein 10 ng

Lane 3:

GST-tagged human Kir1.1/KCNJ1 recombinant protein 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 17 kDa

false

Exposure time: 15s

Unconjugated

Anti-Kir4.1/KCNJ10 antibody [EPR27251-79]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27251-79

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kir4.1 also known as KCNJ10 is an inwardly rectifying potassium channel. It is a protein with a mass of approximately 45 kDa. Kir4.1 facilitates the movement of potassium ions across the cell membrane helping maintain the membrane potential in certain cells. This channel prominently expresses in the brain kidney and inner ear. In glial cells of the central nervous system it plays an important role in maintaining potassium homeostasis. This is important for the correct functioning of neurons and other glial cells.

Biological function summary

The potassium channel Kir4.1 functions to balance potassium levels in the extracellular space which is important for nerve impulse propagation and muscle contraction. Kir4.1 frequently forms a complex with another potassium channel Kir5.1 (KCNJ16). Together these complexes contribute to fine-tuning the electrochemical gradient impacting various cell types. Its distribution and activity affect neuronal signaling renal function and endolymph generation in the ear.

Pathways

Kir4.1 plays essential roles in neural signaling pathways and renal reabsorption processes. This target interacts with proteins like AQP4 in the brain influencing water transport and ion homeostasis. Its functionality is part of the regulatory mechanisms within the distal convoluted tubule of the kidney impacting water and salt balance. These interactions facilitate proper electrical signaling and osmoregulation in the body.

Kir4.1 has associations with disorders such as epilepsy and EAST syndrome. Defects or dysregulation in the Kir4.1 channel can disrupt potassium ion balance which may lead to abnormal neuronal excitability manifesting as epilepsy. Additionally mutations in this channel can contribute to EAST syndrome a condition involving epilepsy ataxia sensorineural deafness and renal tubulopathy. Related proteins like AQP4 can also be implicated highlighting the complexity of physiological processes involving Kir4.1.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be responsible for potassium buffering action of glial cells in the brain (By similarity). Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (By similarity).

See full target information Kir4.1/KCNJ10

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Immunohistochemistry - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Western blot - Anti-Kir4.1/KCNJ10 antibody [EPR27251-79] - BSA and Azide free (AB306551)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise