JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251809

Anti-Kir6.1/KCNJ8 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal Kir6.1/KCNJ8 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KCNJ8 aa 250 to C-terminus.

View Alternative Names

ATP-sensitive inward rectifier potassium channel 8, Inward rectifier K(+) channel Kir6.1, uKATP-1, KCNJ8

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)

Paraffin-embedded human heart muscle tissue stained for Kir6.1/KCNJ8 using ab251809 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)

Paraffin-embedded human liver tissue stained for Kir6.1/KCNJ8 using ab251809 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)

Paraffin-embedded human testis tissue stained for Kir6.1/KCNJ8 using ab251809 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.1/KCNJ8 antibody (AB251809)

Paraffin-embedded human skin tissue stained for Kir6.1/KCNJ8 using ab251809 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KCNJ8 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15842

style
left-column

Complementary Products

Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB52625

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (AB52625)

  • 5
  • 12
Primary Antibodies

AB79171

Anti-Kir6.2/BIR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody (AB79171)

  • 3
  • 1
Primary Antibodies

AB16030

Anti-SOCS3 antibody

Western blot - Anti-SOCS3 antibody (AB16030)

  • 4
  • 31
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kir6.1 also known as KCNJ8 is an integral part of a potassium channel complex. It forms the inwardly rectifying potassium channel (Kir) family that allows potassium ions to flow into cells. The Kir6.1 channel often a part of the larger ATP-sensitive potassium channels (K_ATP channels) regulates cellular membrane potentials. The protein mass of Kir6.1 is approximately 45 kDa. You can find Kir6.1 expressed in various tissues including the heart skeletal muscle and smooth muscle as well as in pancreatic beta cells.
Biological function summary

Kir6.1 plays a significant role in linking cellular energy status to membrane excitability through the modulation of potassium ion conductance. It forms a complex with SUR2 (sulfonylurea receptor 2) subunits collectively constituting the K_ATP channel complex. This channel complex senses changes in intracellular ATP levels and its activity influences insulin secretion in the pancreas and vascular tone in smooth muscle. By coupling cellular metabolic status to the ion channel activity Kir6.1 contributes critically to cellular homeostasis.

Pathways

Kir6.1 channels integrate into several important biological systems. Notably the K_ATP channels participate in the cardiac muscle's ischemic conditioning pathway helping protect cells against nutrient deprivation. These channels also engage in the insulin signaling pathway affecting glucose homeostasis and energy metabolism. The interaction with other proteins like SUR2 and Kir6.2 indicates the complex role Kir6.1 plays in these signaling cascades and its indispensable function in metabolic regulation.

The Kir6.1 channel has associations with cardiovascular diseases such as hypertension due to its involvement in vascular tone regulation. Abnormalities in Kir6.1 function can also contribute to disorders like neonatal diabetes linked to defects in insulin secretion due to channel dysfunction. Kir6.1 has connections with the SUR2 protein in these conditions given their partnership in the K_ATP channel complex. Studying these associations helps understand Kir6.1's influence on disease development and potential therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it (PubMed : 20558321, PubMed : 21836131, PubMed : 24700710, PubMed : 28842488). Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages (PubMed : 20558321, PubMed : 21836131, PubMed : 24700710, PubMed : 28842488). The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium (PubMed : 20558321, PubMed : 21836131, PubMed : 24700710, PubMed : 28842488). Can form a sulfonylurea-sensitive but ATP-insensitive potassium channel with ABCC9 (By similarity).
See full target information KCNJ8
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Gastro hep advances 2:721-732 PubMed39129876

2023

Targeting Host Sulphonyl Urea Receptor 2 Can Reduce Severity of Associated Gastritis.

Applications

Unspecified application

Species

Unspecified reactive species

Sohinee Sarkar,Ghazal Alipour Talesh,Trevelyan R Menheniott,Philip Sutton

Computational and mathematical methods in medicine 2021:7731528 PubMed34373698

2021

Naloxone Protects against Lipopolysaccharide-Induced Neuroinflammation and Microglial Activation via Inhibiting ATP-Sensitive Potassium Channel.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijia Tang,Xiaobao Shao,Jun Wu,Hucheng Chen,Anyu Zhang,Fei Xu,He Ping,Shiwei Li,Chunyan Liu,Yijun Li,Xue Xue,Binbin Yuan

Journal of cellular and molecular medicine 25:3935-3949 PubMed33547878

2021

Kir6.1 improves cardiac dysfunction in diabetic cardiomyopathy via the AKT-FoxO1 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxin Wang,Jing Bai,Peng Duan,Hao Wang,Yang Li,Qinglei Zhu

Cell death & disease 11:1065 PubMed33311442

2020

CircRNA hsa_circRNA_104348 promotes hepatocellular carcinoma progression through modulating miR-187-3p/RTKN2 axis and activating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guanqun Huang,Min Liang,Haiyan Liu,Jianhong Huang,Peiqing Li,Chong Wang,Yidan Zhang,Ye Lin,Xianhan Jiang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com