Anti-Kir6.2/BIR antibody
3
(1 Review)
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(10 Publications)
Rabbit Polyclonal Kir6.2/BIR antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications.
View Alternative Names
ATP-sensitive inward rectifier potassium channel 11, IKATP, Inward rectifier K(+) channel Kir6.2, KCNJ11
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody (AB79171)
Immunohistochemical analysis of Kir6.2/BIR in paraffin embedded human brain tissue using a 1/50 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Kir6.2/BIR antibody (AB79171)
Immunofluorescence analysis of Kir6.2/BIR in HeLa cells, using a 1/500 dilution of ab79171, with (right) or without (left) pre treatment with the immunizing peptide.
- WB
Unknown
Western blot - Anti-Kir6.2/BIR antibody (AB79171)
All lanes:
Western blot - Anti-Kir6.2/BIR antibody (ab79171) at 1/500 dilution
Lane 1:
extracts from HeLa cells treated with heat shock at 20 µg
Lane 2:
extracts from NIH3T3 cells treated with heat shock at 20 µg
Lane 3:
extracts from NIH3T3 cells treated with heat shock at 20 µg with immunizing peptide
Predicted band size: 44 kDa
Observed band size: 44 kDa
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- WB
CiteAb
Western blot - Anti-Kir6.2/BIR antibody (AB79171)
Kir6.2/BIR western blot using anti-Kir6.2/BIR antibody ab79171. Publication image and figure legend from Gong, X. G., Sun, H. M., et al., 2015, Evid Based Complement Alternat Med, PubMed 25610480.
ab79171 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab79171 please see the product overview.
Effects of decoctions on protein levels of SUR1 (a) and Kir6.2 (b) measured by Western blot. Density values for each band were first normalized to loading control (β-actin) and then to the control group. Data are expressed as mean ± SEM (N = 5, *p < 0.05 versus control group, △p < 0.05 versus model group). DBYW : Da-Bu-Yin-Wan; QZS : Qian-Zheng-San; CD : combined decoction.
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Reactivity data
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Kir6.2 functions by coupling cellular metabolic states to electrical activity via its role in the K-ATP channel complex. This complex integrates Kir6.2 with the sulfonylurea receptor SUR1 or SUR2 forming an important connection between cellular metabolism and membrane excitability. Kir6.2 helps manage glucose-induced insulin secretion in pancreatic beta cells contributing to the temporal burst of insulin after meals. Its function in neurons and muscle fibers includes balancing cellular energy levels and physiological processes such as neurotransmitter release and muscle contraction.
Pathways
Kir6.2 plays a significant role in insulin secretion and cardiac muscle contraction pathways. It interacts closely with various proteins including SUR1 in the insulin release pathway and SUR2 in cardiovascular regulation. The activity of Kir6.2 links it with processes such as glucose-stimulated insulin secretion where its modulation significantly impacts the entry of calcium ions through voltage-dependent calcium channels further altering the exocytosis of insulin granules. The reactivity to intracellular ATP levels means Kir6.2 acts as a metabolic sensor influencing these pathways accordingly.
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Target data
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Advanced biology 8:e2400117 PubMed38548667
2024
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Immunity, inflammation and disease 12:e1194 PubMed38501544
2024
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Frontiers in pharmacology 14:1115543 PubMed37180726
2023
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Endocrine connections 12: PubMed36398885
2022
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Unspecified reactive species
The EMBO journal 41:e106825 PubMed35023164
2022
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Unspecified reactive species
Science advances 7: PubMed33741588
2021
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Unspecified reactive species
Cardiovascular drugs and therapy 35:87-101 PubMed33057968
2020
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EBioMedicine 54:102723 PubMed32259713
2020
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Evidence-based complementary and alternative medic 2014:729195 PubMed25610480
2014
Applications
WB
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Unspecified reactive species
Journal of the American College of Cardiology 59:390-6 PubMed22133355
2011
Applications
Unspecified application
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Unspecified reactive species
Product promise
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