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AB291089

Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free)

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Rabbit Recombinant Monoclonal Kir6.2/BIR antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Transfected cell line - Mouse, Transfected cell line - Rat, Rat samples.

View Alternative Names

ATP-sensitive inward rectifier potassium channel 11, Inward rectifier K(+) channel Kir6.2, Kcnj11

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)

This data was developed using ab291074, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T transfe tissue labeling Kir6.2/BIR with ab291074 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag; positive staining on (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab291074 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)

This data was developed using ab291074, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Kir6.2/BIR with ab291074 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the intercalated disc in mouse cardiac muscle. The section was incubated with ab291074 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)

This data was developed using ab291074, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Kir6.2/BIR with ab291074 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the islet in rat pancreas. The section was incubated with ab291074 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)

This data was developed using ab291074, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Kir6.2/BIR with ab291074 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the intercalated disc in rat cardiac muscle (PMID 23066018). The section was incubated with ab291074 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir6.2/BIR antibody [EPR25229-115] (BSA and Azide free) (AB291089)

This data was developed using ab291074, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Kir6.2/BIR with ab291074 at 1/2000 (0.26 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the islet in mouse pancreas (PMID : 24101510). The section was incubated with ab291074 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25229-115

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab291089 is a carrier free version of ab291074.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kir6.2 also known by alternate names KCNJ11 and 2BIR is part of the ATP-sensitive potassium channel (K-ATP channel) complex specifically the pore-forming subunit. This protein has a molecular mass of approximately 43 kDa. Expression of Kir6.2 occurs in various tissues most notably in pancreatic beta cells cardiac muscle and neuronal tissues. These channels play an important role in cell membrane potential regulation and excitability modulating insulin release and muscle contraction by responding to intracellular levels of ATP and ADP.
Biological function summary

Kir6.2 functions by coupling cellular metabolic states to electrical activity via its role in the K-ATP channel complex. This complex integrates Kir6.2 with the sulfonylurea receptor SUR1 or SUR2 forming an important connection between cellular metabolism and membrane excitability. Kir6.2 helps manage glucose-induced insulin secretion in pancreatic beta cells contributing to the temporal burst of insulin after meals. Its function in neurons and muscle fibers includes balancing cellular energy levels and physiological processes such as neurotransmitter release and muscle contraction.

Pathways

Kir6.2 plays a significant role in insulin secretion and cardiac muscle contraction pathways. It interacts closely with various proteins including SUR1 in the insulin release pathway and SUR2 in cardiovascular regulation. The activity of Kir6.2 links it with processes such as glucose-stimulated insulin secretion where its modulation significantly impacts the entry of calcium ions through voltage-dependent calcium channels further altering the exocytosis of insulin granules. The reactivity to intracellular ATP levels means Kir6.2 acts as a metabolic sensor influencing these pathways accordingly.

Kir6.2 is linked to conditions such as neonatal diabetes mellitus and congenital hyperinsulinism. Mutations in the gene encoding Kir6.2 can disrupt normal K-ATP channel functioning leading to these diseases. In neonatal diabetes Kir6.2 alterations impair insulin secretion due to disrupted ATP binding or channel closure while in congenital hyperinsulinism dysfunction of Kir6.2 in hyperactive channels results in excessive insulin production. This exposes strong connections with proteins like insulin within these conditions highlighting its important influence on glucose metabolism and energy homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).
See full target information Kcnj11
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Product promise

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