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Mouse Recombinant Monoclonal Kir6.2/BIR antibody. Suitable for IHC-P and reacts with Rat, Mouse samples.

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Images

Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (AB307371), expandable thumbnail
  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (AB307371), expandable thumbnail
  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (AB307371), expandable thumbnail
  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (AB307371), expandable thumbnail
  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (AB307371), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species

Rat

Dilution info

1/200

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/200

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human

Dilution info

-

Notes

-

Target data

Function

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).

Alternative names

Recommended products

Mouse Recombinant Monoclonal Kir6.2/BIR antibody. Suitable for IHC-P and reacts with Rat, Mouse samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

N363/71

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Kir6.2 also known by alternate names KCNJ11 and 2BIR is part of the ATP-sensitive potassium channel (K-ATP channel) complex specifically the pore-forming subunit. This protein has a molecular mass of approximately 43 kDa. Expression of Kir6.2 occurs in various tissues most notably in pancreatic beta cells cardiac muscle and neuronal tissues. These channels play an important role in cell membrane potential regulation and excitability modulating insulin release and muscle contraction by responding to intracellular levels of ATP and ADP.

Biological function summary

Kir6.2 functions by coupling cellular metabolic states to electrical activity via its role in the K-ATP channel complex. This complex integrates Kir6.2 with the sulfonylurea receptor SUR1 or SUR2 forming an important connection between cellular metabolism and membrane excitability. Kir6.2 helps manage glucose-induced insulin secretion in pancreatic beta cells contributing to the temporal burst of insulin after meals. Its function in neurons and muscle fibers includes balancing cellular energy levels and physiological processes such as neurotransmitter release and muscle contraction.

Pathways

Kir6.2 plays a significant role in insulin secretion and cardiac muscle contraction pathways. It interacts closely with various proteins including SUR1 in the insulin release pathway and SUR2 in cardiovascular regulation. The activity of Kir6.2 links it with processes such as glucose-stimulated insulin secretion where its modulation significantly impacts the entry of calcium ions through voltage-dependent calcium channels further altering the exocytosis of insulin granules. The reactivity to intracellular ATP levels means Kir6.2 acts as a metabolic sensor influencing these pathways accordingly.

Associated diseases and disorders

Kir6.2 is linked to conditions such as neonatal diabetes mellitus and congenital hyperinsulinism. Mutations in the gene encoding Kir6.2 can disrupt normal K-ATP channel functioning leading to these diseases. In neonatal diabetes Kir6.2 alterations impair insulin secretion due to disrupted ATP binding or channel closure while in congenital hyperinsulinism dysfunction of Kir6.2 in hyperactive channels results in excessive insulin production. This exposes strong connections with proteins like insulin within these conditions highlighting its important influence on glucose metabolism and energy homeostasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371), expandable thumbnail

    Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Kir6.2/BIR with ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Positive staining on the islet in rat pancreas.
    The section was incubated with ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371), expandable thumbnail

    Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Kir6.2/BIR with ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Negative control: no staining on the rat liver. 
    The section was incubated with ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371), expandable thumbnail

    Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Kir6.2/BIR with ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Positive staining on the intercalated disc in rat cardiac muscle.
    The section was incubated with ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371), expandable thumbnail

    Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Kir6.2/BIR with ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Positive staining on the intercalated disc in mouse cardiac muscle.
    The section was incubated with ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371), expandable thumbnail

    Immunohistochemistry - Anti-Kir6.2/BIR antibody [N363/71] (ab307371)

    Immunohistochemical analysis of paraffin-embedded cell pellets labeling Kir6.2/BIR with ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Positive staining on (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag; positive staining on (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with empty vector containing a his tag.
    The section was incubated with ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubation with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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