Mouse Recombinant Monoclonal Kir6.2/BIR antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Mouse samples.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IHC-P | ICC/IF | WB | |
---|---|---|---|
Human | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Not recommended |
Rat | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).
ATP-sensitive inward rectifier potassium channel 11, BIR, Inward rectifier K(+) channel Kir6.2, Kcnj11
Mouse Recombinant Monoclonal Kir6.2/BIR antibody. Carrier free. Suitable for IHC-P and reacts with Rat, Mouse samples.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
N363/71
Affinity purification Protein A
Blue Ice
+4°C
+4°C
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Kir6.2 also known by alternate names KCNJ11 and 2BIR is part of the ATP-sensitive potassium channel (K-ATP channel) complex specifically the pore-forming subunit. This protein has a molecular mass of approximately 43 kDa. Expression of Kir6.2 occurs in various tissues most notably in pancreatic beta cells cardiac muscle and neuronal tissues. These channels play an important role in cell membrane potential regulation and excitability modulating insulin release and muscle contraction by responding to intracellular levels of ATP and ADP.
Kir6.2 functions by coupling cellular metabolic states to electrical activity via its role in the K-ATP channel complex. This complex integrates Kir6.2 with the sulfonylurea receptor SUR1 or SUR2 forming an important connection between cellular metabolism and membrane excitability. Kir6.2 helps manage glucose-induced insulin secretion in pancreatic beta cells contributing to the temporal burst of insulin after meals. Its function in neurons and muscle fibers includes balancing cellular energy levels and physiological processes such as neurotransmitter release and muscle contraction.
Kir6.2 plays a significant role in insulin secretion and cardiac muscle contraction pathways. It interacts closely with various proteins including SUR1 in the insulin release pathway and SUR2 in cardiovascular regulation. The activity of Kir6.2 links it with processes such as glucose-stimulated insulin secretion where its modulation significantly impacts the entry of calcium ions through voltage-dependent calcium channels further altering the exocytosis of insulin granules. The reactivity to intracellular ATP levels means Kir6.2 acts as a metabolic sensor influencing these pathways accordingly.
Kir6.2 is linked to conditions such as neonatal diabetes mellitus and congenital hyperinsulinism. Mutations in the gene encoding Kir6.2 can disrupt normal K-ATP channel functioning leading to these diseases. In neonatal diabetes Kir6.2 alterations impair insulin secretion due to disrupted ATP binding or channel closure while in congenital hyperinsulinism dysfunction of Kir6.2 in hyperactive channels results in excessive insulin production. This exposes strong connections with proteins like insulin within these conditions highlighting its important influence on glucose metabolism and energy homeostasis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-Kir6.2/BIR antibody [N363/71] ab307371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Kir6.2/BIR with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Positive staining on the islet in rat pancreas.
The section was incubated with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using Anti-Kir6.2/BIR antibody [N363/71] ab307371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Kir6.2/BIR with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Negative control: no staining on the rat liver.
The section was incubated with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using Anti-Kir6.2/BIR antibody [N363/71] ab307371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Kir6.2/BIR with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Positive staining on the intercalated disc in rat cardiac muscle.
The section was incubated with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using Anti-Kir6.2/BIR antibody [N363/71] ab307371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Kir6.2/BIR with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Positive staining on the intercalated disc in mouse cardiac muscle.
The section was incubated with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubated with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using Anti-Kir6.2/BIR antibody [N363/71] ab307371, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded cell pellets labeling Kir6.2/BIR with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 1/200 dilution (4.63 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Positive staining on (A) HEK-293T transfected with a mouse KCNJ11 expression vector containing a his tag; positive staining on (B) HEK-293T transfected with rat KCNJ11 expression vector containing a his tag. No staining on (C) HEK-293T cells transfected with empty vector containing a his tag.
The section was incubated with Anti-Kir6.2/BIR antibody [N363/71] ab307371 at 4°C overnight. Then incubation with the post primary antibody Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913 at 1/1000 for 8 mins at room temperature, then incubation with the the Goat anti-Rabbit IgG H&L (HRP polymer) secondary antibody at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
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